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第 13 筆
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論文名稱: | 藍點鸚哥魚體內藻膽蛋白之轉變及顏色變化
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研究生: | 黃瑞彥 Jui-Yen Huang
指導教授: | 孫寶年 Bonnie Sun
| 陳麗淑 Li-Shu Chen
學位類別: | 碩士
學校名稱: | 國立臺灣海洋大學
系所名稱: | 食品科學系
學號: | M92320028
畢業學年度: | 93
語文別: | 中文
關鍵字: | 藍點鸚哥魚 parrotfish
| 藻膽蛋白 phycobiliprotein
全文點閱次數: | 5

全文影像連結:

論文全文(pdf)

論文頁數: | 102
摘要: | 藍點鸚哥魚初自海中捕獲解剖時，其腸道後段常呈紅色，在水族缸中飼養數日，腸道之紅色減褪。之前研究將其消化道從食道至直腸依肌肉之型態分為六段，第V段呈鮮紅色，此段腸道水萃取液之吸收光譜，非血紅蛋白，與紅藻水萃取液相似。以共軛焦點顯微鏡觀察到第IV段及第V段腸組織中有螢光物質，紅藻水萃取液亦具螢光，其螢光光譜及Native-PAGE電泳圖，均與紅藻之藻紅蛋白 (phycoerythrin, PE)相似。本研究探討鸚哥魚消化紅藻後，藻紅蛋白的代謝及變化。
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| 石花菜水萃取液的吸收波長在498、545及562 nm，螢光之發射波長在575 nm，萃取液含藻紅蛋白，經40-60 %硫酸銨沈澱分劃後，藻紅蛋白之純度以A565/A280為指標提高至2.1倍，分子量為216 kDa。
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| 藻紅蛋白經trypsin或chymotrypsin作用後，紅色度 (+a)降低，吸收光譜在495 nm (phycourobilin)與565 nm (phycoerythrobilin)會隨作用時間之增加有下降之趨勢，在575 nm之螢光強度亦有下降，以trypsin作用較chymotrypsin強。
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| 藻紅蛋白經藍點鸚哥魚第V段腸道酵素作用後，紅色度 (+a)較控制組低，隨作用時間之增加，吸收光譜在495 nm與565 nm有下降之趨勢，分別較控制組低33.2及62.1 %，於575 nm之螢光強度亦有下降，降低74.8 %，經第IV段腸道酵素作用者則無顯著之差異，推測藻紅蛋白在鸚哥魚第V段腸道中被trypsin及chymotrypsin消化。
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| 藍點鸚哥魚的第IV段及第V段腸道及魚皮含藻藍蛋白及異藻藍蛋白，幼魚餵食紅藻2個月後以螢光光譜觀察得知，於第IV段腸道有藻紅蛋白之發射波峰，且腸道及魚皮中藻藍蛋白及異藻藍蛋白之螢光強度會增加，體色明顯較控制組藍。
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論文目次: | 目錄    vi
| 表目錄    ix
| 圖目錄    ix
| 縮 寫 表    xiii
| 壹、前言    1
| 貳、文獻回顧    3
| 一.鸚哥魚之生理生態    3
| 1.生活史    3
| 2.鸚哥魚之體色    4
| 3.魚貝類紅色素之蓄積及表現    4
| 4.鸚哥魚之食性    6
| 5.消化道之型態    7
| 6.鸚哥魚之消化酶    8
| 二.鸚哥魚攝食海藻的分類及分布    9
| 三.藻膽蛋白之種類及特性    10
| 1.藻紅蛋白    14
| 2.藻藍蛋白    19
| 3.異藻藍蛋白    20
| 四.色素之代謝及轉變    21
| 1.膽色素    21
| 2.藻紅蛋白    24
| 參、實驗架構    25
| 肆、材料與方法    26
| 一.材料    26
| (一)藻類    26
| 1.紅藻    26
| (二)藍點鸚哥魚    26
| 1.幼魚    26
| 2.成魚    26
| 二.方法    29
| (一)藍點鸚哥魚幼魚餵食試驗    29
| (二)飼料製備    29
| (三)魚體藻膽蛋白之萃取    31
| (四)藻紅蛋白之硫酸銨分劃    31
| (五)藻紅蛋白之體外消化試驗    31
| 1.腸道粗酵素液之製備    32
| 2.藻紅蛋白之水解作用    32
| (六)色素之測定    32
| 1.色差    32
| 2.吸收光譜之測定    33
| 3.螢光光譜之測定    33
| 4.藻紅蛋白分子量之測定—Native-PAGE    33
| (七)蛋白質定量    34
| (八)統計分析    34
| 伍、結果與討論    35
| 一、紅藻色素之藻紅蛋白    35
| 1.粗萃取液    35
| 2.硫酸銨沈澱分劃    39
| 二、藻紅蛋白之體外消化    47
| 1.蛋白酶作用    47
| 2.鸚哥魚體腸道之酵素作用    57
| 三、幼魚攝食紅藻對體色素之影響    65
| 1.腸道    65
| 2.魚皮    71
| 陸、結論    77
| 柒、參考文獻    78
|
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