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詳目顯示區塊

第 12 筆
| (以作者名查詢海洋大學圖書館館藏系統)
| (以作者名查詢全國圖書書目資訊網)
| (以作者名查詢全國博碩士系統)
論文名稱: | 吳郭魚脂氧合酶之基因選殖與分析
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研究生: | 高志豪 Chih-Hao Kao
指導教授: | 耿全福 Chiuan-Fu Ken
| 孫寶年 Bonnie Sun Pan
學位類別: | 碩士
學校名稱: | 國立臺灣海洋大學
系所名稱: | 食品科學系
學號: | m92320031
畢業學年度: | 93
語文別: | 中文
關鍵字: | 吳郭魚 tilapia
| 脂氧合酶 lipoxygenase
| 選殖 cloning
全文點閱次數: | 6

全文影像連結:

論文全文(pdf)

論文頁數: | 95
摘要: | 吳郭魚（tilapia）在快速與緩慢升溫及氯化銨緊迫環境中均導致魚鰓、血液脂氧合酶（Lipoxygenase , LOX , 1.13.11.12）活性上升，顯示LOX可作為一種逆境之生物指標。
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| 為了探討LOX活性在逆境增加表現的分子機制，利用緩慢生溫（0.5 ℃/h）將溫度由25 ℃升溫至37 ℃以及將魚體暴露於氯化銨濃度（3 mg/L）中24小時，抽取吳郭魚鰓與肝臟之total RNA，進行反轉錄作用（reverse transcription）合成cDNA，並在cDNA兩端接上人工合成之adaptor，可與BD SMARTTM RACE cDNA Amplification Kit內附之Universal Primer Mix（UPM）接合，以利基因選殖。
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| 利用美國國家生物資訊中心National Center for Biotechnology Information（NCBI）找尋挪威鼠、家鼠、小白鼠、倉鼠、兔子、牛、黑猩猩、人類、斑馬魚、馬鈴薯、黃豆等物種之5-, 12-, 15-LOX序列，以歐洲國家生物資訊中心（EBI）網站，分析保守區域（conserve region），並設計退化性引子（degenerate primer），同時也以該引子互補股作為另一方向之引子，利用Rapid amplification of cDNA ends （RACE）-PCR進行反應。
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| 以吳郭魚鰓cDNA為模板，經Universal Primer Mix（UPM）與TiLOX2F為引子，Annealling temperature 42-48 ℃，得到1000 bp的單一亮帶，經接合選殖載體（cloning vector）pCR2.1，再轉殖入選殖菌體（cloning host）Ecos101，經大量培養後抽取質體（plasmid）DNA，經限制酶（EcoRΙ）切割後，經電泳確認引子夾擠之序列長度無誤，送交定序，定序結果經NCBI與物種間LOX進行序列比對，確定為LOX序列，於848-914 bp與斑馬魚（Danio rerio）12-lipoxygenase mRNA partial sequence有100 ％的相似度。
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論文目次: | 目錄……………………………………………………………………..vi
| 圖次……………………………………………………………………...x
| 中文摘要...................................................................................................ii
| 英文摘要...................................................................................................iv
|
| 壹. 前言    1
| 貳. 文獻回顧    3
| 一. 脂氧合酶之生理功能    3
| （一）脂氧合酶可調控水生無脊椎動物卵母細胞之成熟    3
| （二）Hepoxilin可活化與調節哺乳動物細胞內之鈣離子    3
| （三）脂氧合酶參與植物老化、受傷或疾病之變化    4
| （四）脂氧合酶調控植物之生長與分化    4
| 二. 脂氧合酶之基因選殖    5
| （一）5-脂氧合酶    5
| （二）12-脂氧合酶    5
| （三）15-脂氧合酶    6
| 三. 動植物脂氧合酶生化特性之比較    6
| （一）構形    6
| （二）胺基酸序列    8
| （三）最適作用pH    9
| （四）最適作用溫度    9
| （五）基質特異性    10
| 四. 脂氧合酶活性之測定    10
| （一）氧電極法    10
| （二）吸光度法    10
| （三）高效能液相層析法    11
| （四）化學冷光法    11
| 參. 材料與方法    12
| 一. 吳郭魚    12
| 二. 實驗藥品    12
| 三. 方法    13
| （一）魚體測定    13
| （二）蓄養條件    13
| （三）抽血與離心    13
| （四）組織製備    14
| （五）蛋白質萃取（Total protein extraction）    14
| （六）脂氧合酶（Lipoxygenase, LOX）之基因選殖    14
| 1. Total RNA之抽取    14
| 2. cDNA之合成    15
| 3. 聚合酶反應（Polymerase Chain Reaction, PCR）    17
| 4. RACE（Rapid Amplification cDNA End）    18
| 5. DNA/RNA電泳膠體之製備    18
| 6. 電泳分析    19
| 7. DNA elution    19
| 8. 次選殖（Subcloning）    20
| （1）接合選殖載體，菌體之轉形（transformation）    20
| （2）菌株培養    20
| 9. 質體之製備    21
| 10. 限制酶切割與電泳觀察    21
| 11. 序列之判讀    22
| （七）脂氧合酶之種類鑑定    22
| （八）蛋白質分子量之測定    23
| 1. Native-PAGE（Polyacrylamide gel electrophoresis）    23
| 2. SDS（Sodium dodecyl sulfate）-PAGE    24
| 3. 西方點墨法（Western blotting）    25
| （九）蛋白質定量    25
| 肆. 結果與討論    26
| 一. 實驗方法之討論    26
| （一）各物種間LOX序列之比對及引子（primer）設計    26
| （二）RNA抽取，cDNA合成    34
| （三）聚合酶鏈反映（polymerase chain reaction, PCR）    37
| （四）接合選殖載體（cloning vector）    38
| （五）菌體之轉形（transformation）    38
| （六）質體（plasmid）之製備    39
| （七）限制酶切割及電泳觀察    39
| （八）送交定序    40
| 二. 實驗結果之討論    42
| （一）定序結果之分析    42
| 1. Myosin light chain , Ribonuclease H    42
| 2. Hypothetical protein yafS , Phospholipase D    42
| 3. Inositol 1,4,5-trisphosphate receptor type 3    47
| 4. Tetraodon nigroviridis full-length cDNA , Phage potal protein    47
| 5. Actin , Cytochrome c oxidase    52
| 6. Bacteriophage lambda complete genome    55
| 7. Danio rerio 12-lipoxygenase mRNA partial sequence    55
| （二）脂氧合酶（Lipoxygenase, LOX）家族之變異性與分類    62
| 伍. 結論    64
| 陸. 參考文獻    69
|
參考文獻: | 周秉則。2004。升溫與氯化胺緊迫對吳郭魚血液之特性及血漿中脂氧合酶活性之影響。國立台灣海洋大學食品科學系研究所碩士論文。基隆。
| 陳素鳳。1993。攝食維生素E及魚油對養殖烏魚血液脂氧合酶特性及血液黏度的影響。國立台灣海洋大學食品科學系研究所碩士論文。基隆。
| 許秀華。1996。烏魚脂氧合酶的純化及特性。國立台灣海洋大學食品科學系研究所碩士論文。基隆。
| 劉玉茹。2000。鎘污染及綠茶液對魚血液、鰓脂氧合酶及白三烯素形成之影響。國立台灣海洋大學食品科學系研究所碩士論文。基隆。
| 劉德樂。2003。低溫與氯化胺緊迫對吳郭魚血液之流變性、脂氧合酶及環氧合酶產物之影響。國立台灣海洋大學食品科學系研究所碩士論文。基隆。
| Altschuler, M., Grayburn, W.S., Collin, G.B. and Hildebrand, D.F. 1989. Developmental expression of lipoxygenase in soybeans. Plant Sci. 63：151-158.
| Angerman-Stewart, J. A., Eling, T. E. and Glasgow, W. C. 1995. Prostaglandin H synthase-2 is induced in Syrian hamster embryo cells in response to basic fibroblast growth factor. Arch Biochem Biophys. 2:378-86.
| Anthon, G.E. and Barrett, D.M. 2003. Thermal inactivation of lipoxygenase and hydroperoxytrienoic acid lyase in tomatoes. Food Chem. 81：275-279.
| Babitha, M.P., Prakash, H.S. and Shetty, H.S. 2004. Purification and properties of lipoxygenase induced in downy mildew resistant pearl millet seedlings due to infection with Sclerospora graminicola. Plant Sci. 166：31-39.
| Bachem, C.W.B., van der Hoeven, R.S., de Bruijn, S.M., Vreugdenhil, D., Zabeau, M. and Visser, R.G.F. 1996. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP；Analysis of gene expression during potato tuber development. Plant. J. 9：745-753.
| Balcarek, J. M., Theisen, T. W., Cook, M. N., Varrichio, A., Hwang, S. M., Strohsacker, M. W. and Crooke, S. T. 1988. Isolation and characterization of a cDNA clone encoding rat 5-lipoxygenase. J Biol Chem. 27:13937-13941.
| Baracat-Pereira, M.C., de Almeida Oliveura, M.G., de Barros, E.G., Moreira, M.A. and Santoro, M.M. 2001. Biochemical properties of soybean leaf lipoxygenase：presence of soluble and membrane-bpund forms. Plant Physiol. Biochem. 39：91-98.
| Baracat-Pereira, M.C., de Almeida Oliveira, M.G., de Barros, E.G., Moreira, M.A. and Santoro, M.M. 2001. Biochemical properties of soybean leaf lipoxygenase：Presence of soluble and membrane-bound forms. Plant Physiol. Biochem. 39：91-98.
| Boyington, J. C., Gaffney, B. J. and Amzel, L. M. 1997. The three-dimensional structure of soybean lipoxygenase-1: an arachidonic acid 15-lipoxygenase. Adv. Exp. Med. Biol. 400:133-138.
| Brash, A. R., Hughes, M. A., Hawkins, D. J., Boeglin, W. E., Song, W. C. and Meijer, L. 1991. Allene oxide and aldehyde biosynthesis in starfish oocytes. J Biol Chem. 266:22926-22931.
| Calhoun, W. J. 2001. Anti-Leukotrienes for asthma. Current Opinion in Pharmacology. 1：230-234.
| Carlen, P.L., Gurevich, N., Zhang, L., Wu, P.H., Reynaud, D. and Pace-Asciak, C.R. 1994. Formation and electrophysiological actions of the arachidonic acid metabolites, hepoxilins, at nanomolar concentration in rat hippocampal slices. Neuroscience. 58：493-502.
| Chen, B.K. and Chang, W.C. 2000. Function interaction between c-jun and promoter factor Sp1 in epidermal growth factor-induced gene expression of human 12（s）-lipoxygenase. Biochim Biophys. Acta. 97：19.
| Chen, X.S., Kurre, U., Jenkins, N.A., Copeland, N.G. and Funk, C.D. 1994. platelet-type 12-lipoxygenase and leukocyte-type 12-lipoxygenase. J. Biol. Chem. 269：13979-13987.
| Christopher, J.P., Pistorius, E.K. and Axelrod, B. 1972. Isolation of third isoenzyme of soybean lipoxygenase. Biochim. Biophys. Acta. 284：54-62.
| Chung, L.C. and Liu, I.C. 1989. Test for ammonia toxicity of cultured hybrid tilapia. In：Hirano, R. and I. Hanyu（Eds.）. The Second. Asian Fisheries Forum. Tokyo. Japan. pp. 457-460.
| Clark, M. S., Edwards, Y. J., Elgar, G. and Bishop, M. J. 1998. The identification and characterization of microsatellites in the compact genome of the Japanese pufferfish, Fugu rubripes: perspectives in functional and comparative genomic analyses. J. Mol. Biol. 278:843-54.
| Coffey, M., Phare, S. and Peters-Golden, M. 2004. Induction of inducible nitric oxide synthase by lipopolysaccharide/interferon gamma and sepsis down-regulates 5-lipoxygenase metabolism in murine alveolar macrophages. Exp Lung Res. 7:615-33.
| De Marzo, N., Sloane, D.L., Dicharry, S., Highland, E. and Sigal, E. 1992. Cloning and expression of an airway epithelial 12-lipoxygenase. Am. J. Physiol. 262：L198-L207.
| Dixon, R.A.F., Jones, R.E., Diehl, R.E., Bennett, C.D., Kargman, S. and Rouzer, C.A. 1988. Cloning of the cDNA for human 5-lipoxygenase. Proc. Natl. Acad. Sci. USA. 85：416-420.
| Eskra, J.D., Pereira, M.J. and Ernest, M.J. 1986. Solid-phase extraction and high-performance liquid chromatography analysis of lipoxygenase pathway products. Anal. Biochem. 154：332.
| Ewing, E.E. and Struik, P.C. 1992. Tuber formation in potato：induction, initiation and growth. Hortic. Rev. 14：89-198.
| Ferrie, B.J., Beaudoin, N., Burkhart, W., Bowsher, C.G. and Rohstein, S.J. 1994. The cloning of two tomato lipoxygenase genes and their differential expression during fruit ripening. Plant Physiol. 106：109-118.
| Feussner, I., Hause, B., Nellen, A., Wasternack, C. and Kindi, H. 1996. Lipid body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms. Planta. 198：288-293.
| Fleming, J., Thiele, B.J., Chester, J., O'Prey, J., Janetzki, S., Aitken, A., Anton, I.A., Rapoport, S. M. and Harrison, P. R. 1989. The complete sequence of the rabbit erythroid cell-specific 15-lipoxygenase mRNA. Gene. 79：181-188.
| Fornaroli, S.F., Petrussa, E., Braidot, E., Vianello, A. and Macri, F. 1999. Purification of a plasma membrane-bound lipoxygenase from soybean cotyledons. Plant sci. 145：1-10.
| Freire-Moar, J., Alavi-Nassab, A., Ng, M., Mulkin, M. and Sigal, E. 1995. Cloning and characterization of a murine macrophage lipoxygenase. Biochim. Biophys. Acta. 1254：112-116.
| Fuller, M.A., Weichert, H., Fischer, A.M., Feussner, I. and Grimers, H.D. 2001. Activity of soybean lipoxygenase isoforms against esterified fatty acid indicates function specificity. Archives of Biochem. and Biophys. 388：146-154.
| Funk, C.D., Keeney, D.S., Oliw, E.H., Boeglin, W.E. and Brash, A.R. 1996. Functional expression and cellular localization of a mouse epidermal lipoxygenase. J. Biol. Chem. 271：23338-23344.
| Funk, C.D., Furci, L. and FitzGerald, G.A. 1990. Molecular cloning, primary structure, and expression of the human platelet/ erythroleukemia cell 12-lipoxygenase. Proc. Natl. Acad. Sci. USA. 87：5638-5642.
| Funk, C.D. 1996. The molecular biology of mammalian lipoxygenase and quest for eicosanoid function using lipoxygenase-depficient mice. Biochim Biophys. Acta. 1304：65-84.
| Gardner, H. W. 1991. Recent investigations into the lipoxygenase pathway of plants. Biochim Biophys Acta. 1084:221-39.
| Hada, T., Hagiya, h., Suzuki, H., Arakawa, T., Nakamura, M., Matsuda, S., Yoshimoto, T., Tamamoto, S., Azekawa, T., Morita, Y., Ishimura, K. and Kim, H.-Y. 1994. Arachidonate 12-lipoxygenase of rat pineal glands: catalytic properties and primary structure deduced from its cDNA. Biochim. Biophys. Acta. 1211：221-228.
| Hada, T., Swift, L. L. and Brash, A. R. 1997. Discovery of 5R-lipoxygenase activity in oocytes of the surf clam, Spisula solidissma. Biochim. et Biophys. Acta. 1346：109-119.
| Hamberg, M. and Samuelsson, B. 1967. On the specificity of the oxygenation of unsaturated fatty acid catalyzed by soybean lipoxidase. J. Biol. Chem. 242（22）：5329-5335.
| Hawkins, D. J. and Brash, A. R. 1987. Eggs of the sea urchin, Strongylocentrotus purpuratus, contain a prominent (11R) and (12R) lipoxygenase activity. J Biol Chem. 262:7629-34.
| Hilbers, M.P., Finazzi-Agro, A., Veldink, G.A. and Vliegnthart, J.F.G. 1996. Purification and characterization of a Lentil seedling lipoxygenase expressed in E.coli.：implication for the mechanism of oxodiene formation by lipoxygenase. Int. J. Biochem. Cell Biol. 28：751-760.
| Holman, R.T., Egwin, P.O. and Christie, W.W. 1969. Substrate specificity of soybean lipoxidase. J. Biol. Chem. 244：1149-1151.
| Hoque,A., Lippman, S.M., Wu, T.T., Xu, Y., Liang, Z.D., Swisher, S.,
| Zhang, H., Cao, L., Ajani, J.A. and Xu, X.C. 2005. Increased 5-lipoxygenase expression and induction of apoptosis by its inhibitors in esophageal cancer: a potential target for prevention. Carcinogenesis 26：785-791.
| Hsieh, R.J., German, J.B. and Kinsella, J.E. 1988a. Lipoxygenase in fish tissue：some properties of the 12-lipoxygenase from trout gill. J. Agric. Food Chem. 36：680.
| Hu, S. P., Pan Sun, B. 2000. Modification of fish oil aroma using a macroalgal lipoxygenase. JAOCS. 77：4.
| Hughes, R. K, West, S. I, Hornostaj, A. R, Lawson, D. M, Fairhurst, S. A, Sanchez, R. O, Hough, P, Robinson, B. H. and Casey, R. 2001. Probing a novel potato lipoxygenase with dual positional specificity reveals primary determinants of substrate binding and requirements for a surface hydrophobic loop and has implications for the role of lipoxygenases in tubers. Biochem J. 353:345-55.
| Izumi, T., Hoshiko, S., RÅdmark, O. and Samuelsson, B. 1990. Cloning of cDNA for human 12-lipoxygenase. Proc. Natl. Acad. Sci. 87：7477-7481.
| Jones, S. M., Luo, M., Peters-Golden, M. and Brock Thomas, G. 2003. Identification of two novel nuclear import sequences in the 5-lipoxygenase protein. The j. of boil. chem. 278：10257-10263.
| Joseph, p., Srinvasan, S.N. and Kulkarni, A.P. 1993. Purification and partial characterization of lipoxygenase with dual catalytic activity from human term placenta. Biochem. J. 293：83-91.
| Kato, T., Maeda, Y., Hirukawa, T., Namai, T. and Toshioka, N. 1992. Lipoxygenase activity increment in infected tomato leaves and oxidation product of linolenic acid by its in vitro enzyme reaction. Biosci. Biotech. Biochem.. 56（3）：373.
| Kausch, K.D. and Handa, A.K. 1997. Molecular clonging of a ripening-specific lipoxygenase and its expression during wild-type and mutant tomato fruit decelopment. Plant Physiol. 113：1041-1050.
| Kondo, Y., Miyazawa, T. and Mizutani, J. 1992. Detection and time-couse analysis of phospholipids hydroperoxide in soybean seedlings after treatment with fungal elicitor by chemiluminescence-HPLC assay. Biochim. Biophys. Acta. 1127：227.
| Kuhn, H. and Thiele, B.J. 1999. The diversity of the lipoxygenase family many sequence data but little information on biological significance. Federation of European Biochem. Societies. 449：7-11.
| Kuo, J.M. and Pan, B.S. 1992. Occurrence of properties of 12-lipoxygenase in the hemolymph of shrimp（Penaeus japonicus Bate）. J. Chinese Biol. Soc. 21（1）：9.
| Lilius, E.M. and Laakso, S. 1982. A sensitive lipoxygenase assay based on chemiluminescence. Anal. Biochem. 119：135.
| Liu, C., Xu, D., Sjöberg, J., Forsell, P., Björkholm, M. and Claesson, H.E. 2004. Transcriptional regulation of 15-lipoxygenase expression by promoter methylation. Experimental cell research. 297：61-67.
| Matsui, K., Irie, M., Kajiwara, T. and Hatanaka, A. 1992. Developmental changes in lipoxygenase activity in cotyledons of cucumber seedings. Plant Sci. 85：23-32.
| Matsumoto, T., Funk, C.D., Rådmark, O., Höög, J.O., Jörnvall, H. and Samuelsson, B. 1988. Molecular cloning and amino acid
| sequence of human 5-lipoxygenase. Proc. Natl. Acad. Sci. USA. 85：26-30 and correction on p.3406.
| Matsuyama, M., Yoshimura, R., Mitsuhashi, M., Hase, T., Tsuchida, K.,
| Takemoto, Y., Kawahito, Y., Sano, H. and Nakatani, T. 2004. Expression of lipoxygenase in human prostate cancer and growth
| reduction by its inhibitors. Int. J. Oncol. 24：821-827.
| Meijer, L., Brash, A.R., Bryant, R.W., Ng, K., Maclouf, J. and Sprecher, H. 1986. Stereospecific induction of starfish oocyte maturation by (8R)-hydroxyeicosatetraenoic acid. J. Biol. Chem. 261：17040-17047.
| Melan, M.A., Enriquez, A.L.D. and Peterman, T.K. 1994. The LOX1 gene of Arabidopsis is temporally and spatially regulated in germinating seeding. Plant Physiol. 105：385-393.
| Mitsuda, H., Yasumoto, K., Yamamoto, A. and Kusano, T. 1967. Study of soybean lipoxygenase-1 preparation of crystalline enzyme and assay by polarographic method. Agric. Biol. Chem. 31：115-118.
| Mohri, S., Cho, S.Y., Endo, Y., Fujimoto, K. 1990. Lipoxygenase activity in sardine skin. Agric. Biol. Chem. 54：1889-1891.
| Mohri, S., Cho, S.Y., Endo, Y., Fujimoto, K. 1992. Linoleate 13（S）-lipoxygenase in sardine skin. J. Agric. Food Chem. 40：573-576.
| Nakayama, T., Takeura, Y. and Ueda, T. 1995. Visible spectrophotometric assay purification and molecular properties of a lipoxygenase from eggplant（solanum melongena linne）fruits. Biochem. and Biophy. Res. commun. 214：1067-1072.
| Narumiya, S., Salmon, J. A., Flower, R. J. and Vane, J. R. 1982. Purification and properties of arachidonate-15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes. Adv Prostaglandin Thromboxane Leukot Res. 9:77-82.
| Navaratnam, S., Salmon, J.A., Cottee, F.H., Weatherley, B.C. and Flower, R. 1982. Arachidonic acid 15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes. J. Biol. Chem. 256（18）：9583-9592.
| Nielsen, G. S., Larsen, L. M. and Poll, L. 2004. Formation of volatile compounds in model experiment with crude Leek（Allium ampeloprasum Var. Lancelot）enzyme extract and linoleic acid or linolenic acid. J. Agric. Food chem. 52：2315-2321.
| Pace-Asciak, CR. 1988. Formation and metabolism of hepoxilin A3 by the rat brain. Biochem. Biophys. Res. Commun. 151：493-498.
| Pace-Asciak, CR. 1994. Hepoxilins：a review on their cellular actions. Biochem. Biophys. Acta. 1215：1-8.
| Prigge, ST., Boyington, JC., Faig, M., Doctor, KS., Gaffney, BJ. and Amzel, LM. 1997. Structure and mechanism of lipoxygenase. Biochimie. 79：629-636.
| Ricker, K.E. and Bostock, R.M. 1994. Eicosanoids in the Phytophthora infestans-potato interaction：Lipoxygenase metabolism of arachidonic acid and biological activities of selected lipoxygenase products. Physiol. Mol. Plant Pathol. 44：65-80.
| Rigos, G., Andriopoulou, A. and Nengas, I. 2002. Pharmacokinetics and tissue distribution of oxytetracycline in sea bass, Dicentrarchus labrax, at two water temperature. Aquaculture. 210：59-67.
| Rodriguez-Concepcion, M. and Beltran, J.P. 1995. Repression of the pea lipoxygenase gene loxg is associated with carpel development. Plant Mol. Biol. 27：887-899.
| Rusterucci, C., Montillet, J.L., Agnel, J.P., Battesti, C., Alonso, B., Knoll, A., Bessoule, J.J., Etienne, P., Suty, L., Blein, J.P. and Triantaphylides, C. 1999. Involvement of lipoxygenase-dependent production of fatty acid hydroperoxides in the development of the hypersensitive cell death induced by cryptogein on tobacco leaves. J. Biol. Chem. 274：36446-36455.
| Saravitz, D.M. and Siedow, J.M. 1995. The lipoxygenase isozymes in soybean 【Glycine max（L.）Merr.】leaves：Changes during leaf development. After wounding, and following reproductive sink removal. Plant Physiol. 107：535-543.
| Schneider C. and Brash, A.R. 2002. Lipoxygenase-catalyzed formation of R-configuration hydroperoxides. Prostaglandins＆ other Lipid Mediators. 68：291-301.
| Schwarz, K., Walther, M., Anton, M., Gerth, C., Feussner, I. and K, H. 2001. Structural basis for lipoxygenase specificity conversion of the human leukocyte 5-lipoxygenase to a 15-lipoxygenase enzyme species by site-directed mutagenesis. The j. of Biol.chemistry. 276：773-779.
| Shibata, D., Kato, T. and Tanaka, K. 1991. Nucleotide sequences of a soybean lipoxygenase gene and the short intergenic region between an upstream lipoxygenase gene. Plant Mol Biol. 16(2):353-359.
| Sigal, E., Craik, C.S., Highland, E., Grunberger, D., Costello, L.L., Dixon, R.A.F. and Nadel, J.A. 1988. Molecular cloning and primary structure of human 15-lipoxygenase. Biochem. Biophys. Res. Commun.. 157：454-464.
| Sigal, E. 1991. The molecular biology of mammalian arachidonic acid metabolism. Am. J. Physiol. 260：L13-L28.
| Sigal, E., Dicharry, S., Highland, E. and Finkbeiner, W.E. 1992. Cloning of human airway 15-lipoxygenase: identity to the reticulocyte enzyme and expression in epithelium. Am. J. Physiol. 262：L392-L398.
| Steczko, J., Muchmore, C.R., Smith, J.L. and Axelrod, B. 1990. Crystallization and preliminary x-ray investigation of lipoxygenase-1 from soybeans. J. Biol. Chem. 265：11352-11354.
| Studynkova, J.T., Kuchen, S., Jeisy, E., Schedel, J., Charvat, F., Jarosova, K., Sprott, H., Matucci-Cerinic, M., Gay, R.E., Michel, B.A., Pavelka, K., Vencovsky, J. and Gay, S. 2004. The expression of cyclooxygenase-1, cyclooxygenase-2 and 5-lipoxygenase in inflammatory muscle tissue of patients with polymyositis and dermatomyositis. J. Neural Transm. 22：395-402.
| Surrey, K. 1964. Spectrophotometric method for determination of lipoxygenase activity. Plant Physiol. 39：65-70.
| van, Dijk., K.W., Steketee, K., Havekes, L., Frant, R. and Hofker, M. 1995. Genomic and cDNA cloning of a novel mouse lipoxygenase gene. Biochim Biophys. Acta. 1259：4-8.
| van Mechelen, J.R., Smits, M., Douma, A.C., Rouster, J., Cameron-Mills, V., Heidekamp, F. and Valk, B.E. 1995. Primary structure of a lipoxygenase from barley grain as deduced from its cDNA sequence. Biochim. Biophys. Acta. 1254：221-225.
| Vaughn, S.F. and Gardner, H.W. 1993. Lipoxygenase-derived aldehydes inhibit fungi pathogenic on soybean. J. Chem. Ecol. 19：2337-2345.
| Wallach, D. P. and Brown, V. R. 1981. A novel preparation of human platelet lipoxygenase. Characteristics and inhibition by a variety of phenyl hydrazones and comparisons with other lipoxygenases. Biochim Biophys Acta. 2:361-72.
| Wallet, DP. and Brown, V.R. 1981. A novel preparation of human platelet lipoxygenase. Biochim. Biophys. Acta. 663：361-372.
| Watanabe, T. and Haeggstrom, J. Z. 1993. Rat 12-lipoxygenase: mutations of amino acids implicated in the positional specificity of 15- and 12-lipoxygenases. Biochem Biophys Res Commun. 3:1023-1029.
| Wisniewski, J.P., Gardner, C.D. and Brewin, N.J. 1999. Isolation of lipoxygenase cDNA clones from pea nodule mRNA. Plant Mol. Biol. 39：775-783.
| Yamamoto, S. 1992. Mammalian lipoxygenases: molecular structures and functions. Biochim Biophys. Acta. 1128：117-131.
| Yoshimoto, T., Yamamoto, Y., Arakawa, T., Suzuki, H., Yamamoto, S., Yokoyama, C., Tanabe, T. and Toh, H. 1990. Molecular cloning and expression of human arachidonate 12-lipoxygenase. Biochem. Biophys. Res. commun. 172：1230-1235.
| Yoshimoto, T., Suzuki, H., Yamamoto, S., Takai, T., Yokoyama, C. and Tanabe, T. 1990. Cloning and sequence analysis of the cDNA for arachidonate 12-lipoxygenase of porcine leukocytes. Proc. Natl. Acad. Sci. USA. 87：2142-2146.
| Zhang, C.H., Hirano, T., Suzuki, T. and Shirai, T. 1992a. Enzymatically generated specific volatile compounds in ayu tissues. Nippon Suisan Gakkaishi. 58（3）：559.
| Zhang, Y. Y., Radmark, O. and Samuelsson, B. 1992b. Mutagenesis of some conserved residues in human 5-lipoxygenase：effect on enzyme activity. Proc. Natl. Acad. Sci. USA. 89：485-489.
| Zhang, C.H., Shirai, T., Suzuki, T. and Hirano, T. 1992c. Lipoxygenase-like activity and formation of characteristic aroma compounds from wild and cultured ayu. Nippon Suisan Gakkaishi. 58：959-964.
| Zhao, L. and Funk, C. D. 2004. Lipoxygenase pathway in atherogenesis. Trends. Cardiovasc. Med. 14：191-195.
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2009 年 11 月 22 日 23:33