:::

| | | | | | 第 | | 筆

詳目顯示區塊

第 7 筆
| (以作者名查詢海洋大學圖書館館藏系統)
| (以作者名查詢全國圖書書目資訊網)
| (以作者名查詢全國博碩士系統)
論文名稱: | 馬尾藻生質乙醇之最適生產條件探討
| Study on the Optimal Production Conditions of Bioethanol from Sargassum siliquosum
研究生: | 吳奕霈 Yi-Pei Wu
指導教授: | 潘崇良 Chorng-Liang Pan
學位類別: | 碩士
學校名稱: | 國立臺灣海洋大學
系所名稱: | 食品科學系
學號: | 19932013
畢業學年度: | 100
語文別: | 中文
關鍵字: | 馬尾藻 Sargassum siliquosum
| 生質乙醇 Bioethanol
全文影像連結: | | (本論文20150730對校內公開)
| 論文全文(pdf)

論文頁數: | 92
摘要: | 15 g 乾燥馬尾藻經 121℃ 熱水萃取處理 40 min 後，以 0.4 N 鹽酸、13,680 U 纖維素酶、及 100 U 馬尾藻多醣所誘導 MA103 與 MAEF108 之粗酵素液的順序進行水解，經 12% 酵母菌 BCRC 21686 + 21687 + 22220 於 18℃ 發酵 3 day，可初步得到 18.15 g/100 g 之 乙醇產率。酵母菌耐鹽性試驗顯示酵母菌 BCRC 21824 與 BCRC 22510 皆具有鹽度 60 psu 之耐鹽性，且此二株菌株的組合能有效利用馬尾藻水解液中之簡單醣類 (21.12 mg/mL)，發酵至殘糖量剩 4.75 mg/mL，產生 1.06% 的乙醇濃度。此外，此酵母菌菌株組合的最適總接種量與最適發酵溫度分別為 12% 與 25℃。15 g 乾燥馬尾藻經 121℃ 熱水萃取處理 20 min 後，以 0.4 N 鹽酸、13,680 U 纖維素酶、22,000 U 澱粉酶及 100 U 馬尾藻多醣所誘導 MA103 與 MAEF108 之粗酵素液的順序進行水解，經 12% 酵母菌 BCRC 21824 + 22510 於 25℃ 發酵 3 day，可進一步得到 21.90 g/100 g 之乙醇產率。另外，15 g 乾燥馬尾藻經 121℃ 熱酸 (0.4 N HCl) 萃取處理 20 min 後，以 13,680 U 纖維素酶、22,000 U 澱粉酶及 100 U 馬尾藻多醣所誘導 MA103 與 MAEF108 之粗酵素液的順序進行水解，經 12% 酵母菌 BCRC 21824 + 22510 於 25℃ 發酵 3 day，最終可得到 25.48 g/100 g 之乙醇產率。饋料批次發酵結果顯示馬尾藻水解液經 12% 酵母菌 BCRC 21824 + 22510 於 25℃ 二次饋料批次發酵 5 day，能使乙醇產率由 25.48 g/100 g 提升至 26.67 g/100 g，深具未來發展潛力。高效能液相層析圖顯示馬尾藻多醣經熱酸萃取後，再以纖維素酶、澱粉酶、及馬尾藻多醣所誘導 MA103 與 MAEF108 之粗酵素液水解後，能將萃取出來的馬尾藻寡醣水解成馬尾藻簡單醣類，並被酵母菌 BCRC 21824 + 22510 利用來發酵生產乙醇。
| 15 g (dry weight) S. siliquosum sequentially treated with 121℃ hot water extraction for 40 min, 0.4 N HCl hydrolysis, 13,680 U cellulase hydrolysis, 100 U MA103 and MAEF108-Sar crude enzymes hydrolysis, then inoculated 12% yeast strains BCRC 21686 + 21687 + 22220 fermentated at 18℃ for 3 day, initially obtain 18.15 g/100 g of ethanol yield. Yeast salt tolerance test have shown that yeast strains BCRC 21824 + 22510 both can tolerate salinity (60 psu), and a combination of this two yeast strains can efficient using the simple sugars in the S. siliquosum hydrolyzate (21.12 mg/mL) to produce 1.06% of ethanol concentration (residual sugar, 4.75 mg/mL). Furthermore, the optimal total inoculation amount and the optimal fermentation temperature of this yeast combination are 12% and 25℃, respectively. 15 g (dry weight) S. siliquosum sequentially treated with 121℃ hot water extraction for 20 min, 0.4 N HCl hydrolysis, 13,680 U cellulase hydrolysis, 22,000 U amylase hydrolysis, 100 U MA103 and MAEF108-Sar crude enzymes hydrolysis, then inoculated 12% yeast strains BCRC 21824 + 22510 fermentated at 25℃ for 3 day, further obtain 21.90 g/100 g of ethanol yield. In addition, 15 g (dry weight) S. siliquosum sequentially treated with 121℃ hot HCl (0.4 N) extraction for 20 min, 13,680 U cellulase hydrolysis, 22,000 U amylase hydrolysis, 100 U MA103 and MAEF108-Sar crude enzymes hydrolysis, then inoculated 12% yeast strains BCRC 21824 + 22510 fermentated at 25℃ for 3 day, finally obtain 25.48 g/100 g of ethanol yield. The fed-batch fermentation test have shown that ethanol fermentation of S. siliquosum hydrolyzate by yeast strains BCRC 21824 + 22510 at 25℃ for 5 day, can make the ethanol yield of 25.48 g/100 g increased to 26.67 g/100 g, this have tremendous potential for future development. The high performance liquid chromatography have shown that S. siliquosum sequentially treated with hot HCl extraction, cellulase hydrolysis, amylase hydrolysis, MA103 and MAEF108-Sar crude enzymes hydrolysis can let the extracted S. siliquosum oligosaccharides hydrolysis into simple sugars, which could be used to fermentated to produce ethanol by yeast strains BCRC 21824 + 22510.
論文目次: | 目錄                    i
| 表目錄                    v
| 圖目錄                    vii
| 附錄目錄                    ix
| 中文摘要                    x
| 英文摘要 (Abstract)            xi
| 壹、前言                    1
| 貳、文獻整理                3
| 一、生質能源                3
| 二、生質乙醇                4
| 2-1. 生質乙醇之特性            4
| 2-2. 生質乙醇之原料            4
| 2-3. 藻類生產生質乙醇之優勢        5
| 2-4. 藻類生產生質乙醇之製程        6
| 2-5. 藻類生產生質乙醇之現況        8
| 2-6. 國內外生質乙醇之市場需求        9
| 三、馬尾藻 (Sargassum sp.)        10
| 3-1. 馬尾藻之生態特性            10
| 3-2. 馬尾藻之組成成分            11
| 四、褐藻膠裂解酶 (alginate lyase)        12
| 4-1. 褐藻膠之結構組成            12
| 4-2. 褐藻膠裂解酶之生產菌株        12
| 4-3. 褐藻膠裂解酶之生化特性        13
| 五、酵母菌                14
| 5-1. 酵母菌之營養需求            14
| 5-2. 酵母菌菌種                15
| 5-3. 乙醇發酵之生化反應            15
| 參、實驗設計                17
| 肆、實驗材料與方法                18
| 一、實驗材料                18
| 1-1. 原料                18
| 1-2. 實驗菌株                18
| 1-2-1. 褐藻膠裂解酶生產菌株        18
| 1-2-2. 酵母菌菌株                18
| 1-3. 試驗藥品                18
| 1-3-1. 藥品                18
| 1-3-2. 酵素                19
| 1-3-3. 培養基組成                20
| 1-3-4. 褐藻膠裂解酶酵素活性反應基質        21
| 1-3-5. 褐藻膠裂解酶酵素活性反應試劑        21
| 1-3-6. 還原醣量反應試劑            22
| 1-4. 儀器設備                22
| 二、實驗方法                24
| 2-1. 馬尾藻之前處理            24
| 2-2. 馬尾藻之一般成分分析            24
| 2-2-1. 水分含量                24
| 2-2-2. 灰分含量                24
| 2-2-3. 粗蛋白含量                25
| 2-2-4. 粗脂肪含量                25
| 2-2-5. 粗纖維含量                25
| 2-3.    MAEF108 與 MA103 粗酵素液之生產    26
| 2-3-1. 菌株保存                26
| 2-3-2. 菌株活化                26
| 2-3-3. 馬尾藻多醣誘導之粗酵素液製備        26
| 2-4. 不同水解順序處理馬尾藻之發酵試驗    27
| 2-4-1. 馬尾藻熱萃多醣液之製備        27
| 2-4-2. 馬尾藻多醣水解液之製備        27
| 2-4-3. 馬尾藻多醣水解液之發酵        28
| 2-5. 酵母菌菌株耐鹽性試驗及醣類利用性篩選    29
| 2-5-1. 菌株保存                29
| 2-5-2. 菌株活化                29
| 2-5-3. 耐鹽性試驗                29
| 2-5-4. 馬尾藻水解液簡單醣類利用性篩選    29
| 2-6. 酵母菌菌株組合之最適乙醇發酵條件探討    30
| 2-6-1. 接種菌量探討            30
| 2-6-2. 發酵溫度探討            30
| 2-7. 不同熱水萃取條件處理馬尾藻之發酵試驗    30
| 2-7-1. 馬尾藻多醣水解液之製備        30
| 2-7-2. 馬尾藻多醣水解液之發酵        32
| 2-8. 不同酸水解條件處理馬尾藻之發酵試驗    32
| 2-8-1. 馬尾藻多醣水解液之製備        32
| 2-8-2. 馬尾藻多醣水解液之發酵        34
| 2-9. 馬尾藻多醣水解液之饋料批次發酵試驗    34
| 2-10. 馬尾藻水解與發酵期間之醣類組成分析    35
| 2-11. 分析方法                35
| 2-11-1. 總醣量測定            35
| 2-11-2. 還原醣量測定            35
| 2-11-3. 褐藻膠裂解酶酵素活性測定        36
| 2-11-4. 酵母菌菌量測定            36
| 2-11-5. 乙醇濃度測定            36
| 2-11-6. 乙醇產率計算            37
| 三、統計分析                37
| 伍、結果討論                38
| 一、馬尾藻之一般成分分析            38
| 二、不同水解順序處理馬尾藻之發酵試驗        38
| 三、酵母菌菌株耐鹽性試驗及醣類利用性篩選    40
| 1. 酵母菌耐鹽性試驗            40
| 2. 馬尾藻簡單醣類利用性篩選        41
| 四、酵母菌菌株組合之最適乙醇發酵條件探討    41
| 1. 最適酵母菌菌株組合            41
| 2. 最適酵母菌總接種量            42
| 3. 最適乙醇發酵溫度            42
| 五、不同熱水萃取條件處理馬尾藻之發酵試驗    43
| 六、不同酸水解條件處理馬尾藻之發酵試驗    44
| 七、馬尾藻多醣水解液之饋料批次發酵試驗    45
| 八、馬尾藻水解與發酵期間之醣類組成分析    46
| 陸、結論                    48
| 柒、參考文獻                50
|
參考文獻: | 古森本。2008。生質能源作物之開發與潛力。農業生技產業。13: 46-53。
| 江福松、呂麗蓉。2012。美國海洋生質能源產業發展現況與趨勢。我國海洋生質能源產業發展趨勢學術研討會，國立臺灣海洋大學主辦，2012 年 3 月 2 日，基隆，臺灣。http://meet.ntou.edu.tw/ 0302www/index.html。
| 吳志偉。2012。我國再生能源發展之現況與願景。我國海洋生質能源產業發展趨勢學術研討會，國立臺灣海洋大學主辦，2012 年 3 月 2 日，基隆，臺灣。http://meet.ntou.edu.tw/0302www/index.html。
| 吳紹祺。1999。海洋菌所產 Agarase 生產條件與海洋多醣經 Agarase 分解所得寡醣類組成之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 吳紹祺。2005。海洋多醣洋菜酶水解物及其發酵產物之生理活性研究。國立臺灣海洋大學食品科學系博士學位論文，基隆，臺灣。
| 林祐生、李文乾。2009。生質酒精。科學發展。433: 20-25。
| 林慧傑。2010。Pseudomonas vescicularis MA103 所產 Alginate lyase pal III 之基因轉殖至大腸桿菌中表現之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 范繼中、李雅琳、吳純衡。2008。海洋綠金-利用海藻生產生質酒精。農政與農情。190: 83-85。
| 范繼中、吳建威、藍惠玲、吳純衡。2007。以海藻產製生質酒精之優勢。水試專訊。19: 24-26。
| 陳文恆、郭家倫、黃文松、王嘉寶。2007。纖維酒精技術之發展。農業生技產業。9: 62-69。
| 陳衍昌。2012。藻類的保種、培養及應用研發。我國海洋生質能源產業發展趨勢學術研討會，國立臺灣海洋大學主辦，2012 年 3 月 2 日，基隆，臺灣。http://meet.ntou.edu.tw/0302www/index.html。
| 許文浩。2002。臺灣酒類發酵微生物之研究。食品工業。34: 20-26。
| 黃俊翰、陳紫媖、蘇惠美。2008。藻類生質能源-回顧與前瞻。水試專訊。21: 29-32。
| 黃淑芳。2004。臺灣海藻資訊網-莢托馬尾藻。國立臺灣博物館，臺北，臺灣。
| 楊依珊。2011。Pseudomonas vescicularis MA103 所產 Alginate lyase 之基因轉殖至 Lactococcus lactis NZ3900 表現及 Alginate lyase 所產寡醣產物分析和用於酵母菌發酵生產乙醇之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 蔡文慶。2010。生質酒精的生產與發展現況。私立朝陽科技大學應用化學系碩士學位論文，臺中，臺灣。
| 劉英俊。1996。最新微生物應用工業。中央圖書出版社，臺北，臺灣。
| 潘崇良。2010。利用藻類生產生質能源。科學發展。448: 26-32。
| 潘崇良。2012。大型海藻生質能源研發之現況與展望。我國海洋生質能源產業發展趨勢學術研討會，國立臺灣海洋大學主辦，2012 年 3 月 2 日，基隆，臺灣。http://meet.ntou.edu.tw/0302www/index.html。
| 潘崇良、陳衍昌、蔡震壽、方翠筠、黃意真、鄒文雄、林富邦、劉秀美、唐世杰。2009。海洋功能性醣體研究：藻類多醣生質乙醇與丁醇及碳交易附加收入。第三屆海峽兩岸魚類生理與養殖研討會，國立臺灣海洋大學水產生物科技頂尖研究中心主辦，2009 年 10 月 19-21 日，基隆，臺灣。
| 賴宜甄。2006。藻類多醣體應用於化妝品之抗老化分析。私立嘉南藥理科技大學化妝品應用與管理系碩士學位論文，臺南，臺灣。
| 蕭斯欣。2012。我國海洋生質能源產業的機會與挑戰。我國海洋生質能源產業發展趨勢學術研討會，國立臺灣海洋大學主辦，2012 年 3 月 2 日，基隆，臺灣。http://meet.ntou.edu.tw/0302www/index.html。
| 蘇惠美、黃俊翰、蔡健偉、陳紫媖。2009。海藻的利用與養殖。科學發展。433: 12-19。
| Adams, J. M., Gallagher, J. A. and Donnison, L. S. 2009. Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments. Applied Phycology 21: 569-574.
| An, Q. D., Zhang, G. L., Wu, H. T., Zhang, Z. C., Gong, W., Liu, Y., Li, X. Z. and Murata, Y. 2008. Production and partial properties of alginase from newly isolated Flavobacterium sp. LXA. Process Biochemistry 43: 842-847.
| AOAC. 1997. Offical Methods of Analysis. 16th edition. Association of Official Analytical Chemists. Washington, DC, U.S.A.
| Aresta, M., Dibenedetto, A. and Barberio G. 2005. Utilization of macro-algae for enhanced CO2 fixation and biofuels production: Development of a computing software for an LCA study. Fuel Processing Technology 86: 1679-1693.
| Baron, A. J., Wong, T. Y., Hicks, S. J., Gacesa, P., Willcock, D. and McPherson, M. J. 1994. Alginate lyase from Klebsiella pneumoniae subsp. aerogenes: Gene cloning, sequence analysis and high-level production in Escherichia coli. Gene 143: 61-66.
| Bhat, M. K. and Bhat, S. 1997. Cellulose degrading enzymes and their potential industrial applications. Biotechnology Advances 15: 583-620.
| Bisaria, V. S. and Mishra, S. 1989. Regulatory aspects of cellulase biosynthesis and secretion. Critical Reviews in Biotechnology 9: 61-103.
|
| Bisaria, V. S. and Ghose, T. K. 1981. Biodegradation of cellulosic materials-substrates, microorganisms, enzymes and products. Enzyme and Microbial Technology 3: 90-104.
| Boulton, R. B., Singleton, V. L., Bisson, L. F. and Kundee, R. E. 1996. Principles and Practices of Winemaking. Chapman and Hall, New York City, NY, U.S.A.
| Cao, L., Xie, L., Xue, X., Tan, H., Liu, Y. and Zhou, S. 2007. Purification and characterization of alginate lyase from Streptomyces sp. strain A5 isolated from banana rhizosphere. Agricultural and Food Chemistry 55: 5113-5117.
| Davis, T. A., Lianes, F., Volesky, B., Diaz-Pulido, G., McCook, L. and Mucci, A. 2003. 1H-MNR study of Na alginates extracted from Sargassum spp. in relation of metal biosorption. Applied Biochemistry and Biotechnology 100: 75-90.
| Dismukes, G. C., Carrieri, D., Bennette, N., Ananyev, G. M. and Posewitz, M. C. 2008. Aquatic phototrophs: efficient alternatives to land-based crops for biofuels. Current Opinion in Biotechnology 19: 235-240.
| Dubois, M., Gillles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28: 350-356.
| Ertesvag, H., Erlien, F., Skjak-Bræk, G., Rehm, B. H. A. and Valla, S. 1998. Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase. Bacteriology 180: 3779-3784.
|
| Fu, X. T., Lin, H. and Kim, S. M. 2007. Purification and characterization of a Na+/K+ dependent alginate lyase from tuban shell Vibrio sp. YKW-34. Enzyme and Microbial Technology 41: 828-834.
| Gombotz, W. R. and Wee, S. F. 1998. Protein release from alginate matrices. Advance Drug Delivery Reviews 31: 267-285.
| Harris, P. 1990. Food Gels. Elsevies Science Publishing Co., Inc., New York City, NY, U.S.A.
| Harun, R., Danquah, M. K. and Forde, G. M. 2010. Microalgal biomass as a fermentation feedstock for bioethanol production. Journal of Chemical Technology and Biotechnology 85: 199-203.
| Heyraud, A., Colin-Morel, P., Girond, S., Richard, C. and Kloareg, B. 1996. HPLC analysis of saturated or unsaturated oligoguluronates and oligomannuronates. Application to the determination of the action pattern of Haliotis tuberculata alginate lyase. Carbohydrate Research 29: 115- 126.
| Horn, S. J. 2000. Bioenergy from brown seaweeds. Ph. D. Dissertation, Department of Biotechnology, Norwegian University of Science and Technology NTNU, Trondheim, Norway.
| Horn, S. J., Aasen, I. M. and Ostgaard, K. 2000a. Ethanol production from seaweed extract. Industrial Microbiology and Biotechnology 25: 249-254.
| Horn, S. J., Aasen, I. M. and Ostgaard, K. 2000b. Production of ethanol from mannitol by Zymomonas palmae. Industrial Microbiology and Biotechnology 24: 51-57.
|
|
| Iwamoto, T., Araki, R., Iriyama, K. I., Oda, T., Fukuda, H., Hayashida, S. and Muramatsu, T. 2001. Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No. 272 and its action on saturated oligomeric substrates. Bioscience Biotechnology and Biochemistry 65: 133-142.
| John, R. P., Anisha, G. S., Nampoothiri, K. M. and Pandey, A. 2011. Micro and Macroalgal biomass: A renewable source for bioethanol. Bioresource Technology 102: 186-193.
| Kasim, N. S., Tsai, T. H., Gunawan, S. and Ju, Y. H. 2009. Biodiesel production from rice bran oil and supercritical methanol. Bioresource Technology 100: 2399-2403.
| Keikhosro, K., Giti, E. and Mohammad, J. T. 2006. Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme and Microbial Technology 40: 138-144.
| Kim, H. and Choi, B. 2010. The effect of biodiesel and bioethanol blended diesel fuel on nanoparticles and exhaust emissions from CRDI diesel engine. Renewable Energy 35: 157-163.
| Kim, N. J., Li, H., Jung, K., Chang, H. N. and Lee, P. C. 2011. Ethanol production from marine algal hydrolysates using Escherichia coli KO11. Bioresource Technology 102: 7466-7469.
| Lebeau, T., Jouenne, T. and Junter, G. A. 1998. Continuous alcoholic fermentation of glucose/xylose mixtures by co-immobilized Saccharomyces cerevisiae and Candida shehatae. Applied Microbiology and Biotechnology 50: 309-313.
| Li, X., Dang, X., Zhao, C., Chen, Z. and Chen, F. 2003. Isolation and some properties of cellulose-degrading Vibrio sp. LX-3 with agar-liquefying ability from soil. World Journal of Microbiology and Biotechnology 19: 375-379.
| Liang, W. S., Yang, Y. S., Su, M. T., Tsai, P. H., Chen, P. H., Wu, C. H. and Pan, C. L. 2011. From Sargassum cristaefolium to renewable bioethanol. 1st International Conference on Algal Biomass, Biofuel and Bioproducts. P3.37. July 17-20, 2011. St. Louis, MO, U.S.A.
| Lynd, L. R., Weimer, P. J., van Zyl, W. H. and Pretorius, I. S. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiology and Molecular Biology Reviews 66: 506-577.
| Mansfield, S. D., Mooney, C. and Saddler, J. N. 1999. Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnology Progress 15: 804-816.
| Matsubara, Y., Iwasaki, K. and Muramatsu, T. 1998. Action of poly (-L-guluronate) lyase from Corynebacterium sp. ALY-1 strain on saturated oligoguluronates. Bioscience Biotechnology and Biochemistry 62: 1055-1060.
| Matsubara, Y., Kawada, R., Iwasaki, K., Kimura, Y., Oda, T. and Muramatsu, T. 2000. Cloning and sequence analysis of a gene (aly PG) encoding poly (α-L-guluronate) lyase from Corynebacterium sp. strain ALY-1. Bioscience and Bioengineering 89: 199-202.
| Matsumoto, M., Yokouchi, H., Suzuki, N., Ohata, H. and Matsunaga, T. 2003. Saccharification of marine microalgae using marine bacteria for ethanol production. Applied Biochemistry and Biotechnology 105: 247-254.
| Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for reagent for deterimination of reduceing sugars. Analytical Chemisry 31: 426-428.
| Miyazaki, M., Obata, J., Iwamoto, Y., Oda, T. and Muramatsu, T. 2001. Calcium-sensitive extracellular poly (-L-guluronate) lyase from a marine bacterium Pseudomonas sp. strain F6: Purification and some properties. Fisheries Science 67: 956-964.
| Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y. Y., Holtzapple, M. and Ladisch, M. 2005. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technology 96: 673-686.
| Muramatsu, T., Yamada, K., Date, M. and Yoshioka, S. 1993. Action of poly (-D-mannuronate) lyase from Turbo cornutus on oligomeric substrates. Bioscience Biotechnology and Biochemistry 57: 1990-1994.
| Ou, Y. F., Yin, P. H. and Zhao, L. 2006. Determination of monosaccharides in Sargassum hemiphyllum (Turner) C. Ag. polysaccharides by ion chromatography. Chinese Journal of Chromatography 24(4): 411-413.
| Park, J. H., Hong, J. Y., Jang, H. C., Oh, S. G., Kim, S. H., Yoon, J. J. and Kim, Y. J. 2012. Use of Gelidium amansii as a promising resource for bioethanol: A practical approach for continuous dilute-acid hydrolysis and fermentation. Bioresource Technology 108: 83-88.
| Preiss, J. and Ashwell, G. 1962. Alginic acid metabolism in bacteria. Biological Chemisrty 237: 309-316.
| Preston, L. A., Bender, C. L. and Schiller, N. L. 2001. Analysis and expression of algL, which encodes alginate lyase in Pseudomonas syringae Pv. syringae. DNA Sequence 12: 455-461.
| Rahman, M. M., Inoue, A., Tanaka, H., and Ojima, T. 2010. Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai. Biochemistry and Molecular Biology 157: 317-325.
| Rioux, L. E., Turgeon, S. L. and Beaulieu, M. 2007. Characterization of polysaccharides extracted from brown seaweeds. Carbohydrate Polymers 69: 530-537.
| Rose, A. H. 1977. Scientific basis of alcoholic beverage production. Economic Microbiology 1: 10-40.
| SAS (Satistical Analytical System). 1999. SAS Use’s: Basic Statistical Analysis. SAS Institute Inc., Cary, NC, U.S.A.
| Sugano, Y., Terada, I., Arita, M., Mona, M. and Matsumoto, T. 1993. Purification and characterization of new agarase from marine bacterium, Vibrio sp. strain JT0107. Applied and Environmental Microbiology 59: 1549-1554.
| Sun, Y. and Cheng, J. Y. 2002. Hydrolysis of lignocellulosic materials for ethanol production: A review. Bioresource Technology 83: 1-11.
| Tan, K. T., Lee, K. T. and Mohamed, A. R. 2008. Role of energy policy in renewable energy accomplishment: The case of second-generation bioethanol. Energy Policy 36: 3360-3365.
| Torres, M. R., Sousa, A. P. A., Silva Filho, E. A. T., Melo, D. F., Feitosa, J. P. A., de Paula R. C. M. and Lima, M. G. S. 2007. Extraction and physicochenical characterization of Sargassum vulgare alginate from Brazil. Carbohydrate Research 342: 2067-2074.
| Wang, M. L., Wang J. T. and Choong Y. M. 2004. Simultaneous quantification of methanol and ethanol in alcoholic beverage using a rapid gas chromatographic method coupling with dual internal standards. Food Chemistry 86: 609-615.
|
| Wang, T. Y., Preston, L. A. and Schiller, N. L. 2000. Alginate lyase: Review of major sources and enzyme characterization, structure- function analysis, biological roles, and applications. Annual Review of Microbiology 54: 289-340.
| Wang, X., Liu, X. and Wang, G. 2011. Two-stage hydrolysis of invasive algal feedstock for ethanol fermentation. Journal of Integrative Plant Biology 53: 246-252.
| Wang, Y. H., Yu, G. L., Wang, X. M., Lv, Z. H., Zhao, X., Wu, Z. H. and Ji, W. S. 2006. Purification and characterization of alginate lyase from marine Vibrio sp. YWA. Acta Biochimica et Biophysica Sinica 38: 633-638.
| Wargacki, A. J., Leonard, E., Win, M. N., Regitsky, D. D., Santos, C. N. S., Kim, P. B., Cooper, S. R., Raisner, R. M., Herman, A., Sivitz, A. B., Lakshmanaswamy, A., Kashiyama, Y., Baker, D. and Yoshikuni Y. 2012. An engineered microbial platform for direct biofuel production from brown macroalgae. Science 335: 307-313.
| Weissbach, A. and Hurwitz, J. 1958. The formation of 2-keto-3- deoxyheptonic acid in extracts of Escherichia coli B. Biological Chemistry 234: 705-709.
| Ye, H., Zhou, C., Liu, J. and Zeng, X. 2008. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chemistry 111: 428-432.
| Yeon, J. H., Lee, S. E., Choi, W. Y., Kang, D. H., Lee, H. Y. and Jung, K. H. 2011. Repeated-batch operation of surface-aerated fermentor for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum. Journal of Microbiology and Biotechnology 21(3): 323-331.
| Yazdani, P., Karimi, K. and Taherzadeh, M. J. 2011. Improvement of enzymatic hydrolysis of a marine macro-alga by dilute acid hydrolysis pretreatment. Bioenergy Technology-World Renewable Energy Congress 2011: 186-191.
|

|

預覽及輸出檢索結果

記錄

欄位

| 本頁 1 筆
| 記錄 7 至 50
| 選擇之記錄

| 標題
| 全部欄位
| selected fields new window
| APA Style
| Chicago Style
| MLA Style
| CNS-13611 Style
| EndNote Style

e-mail 地址 | : |
e-mail 標題 | : |

| | |

系統規劃：海洋大學圖書館
系統製作：飛資得資訊有限公司

2012 年 11 月 19 日 8:48