:::

| | | | | | 第 | | 筆

詳目顯示區塊

第 4 筆
| (以作者名查詢海洋大學圖書館館藏系統)
| (以作者名查詢全國圖書書目資訊網)
| (以作者名查詢全國博碩士系統)
論文名稱: | 高硫酸基含量海藻寡醣之抗凝血與抗血小板凝集和抗病毒活性及被Caco-2 細胞吸收效果之探討
| Study on the Anti-thrombin, Anti-platelet Aggregation, and Antiviral Activity of Oversulfated Marine Algal Oligosaccharides and Its Adsorption Performance with Caco-2 Cell
研究生: | 楊騏瑋 Chi-Wei Yang
指導教授: | 潘崇良 Chorng-Liang Pan
學位類別: | 碩士
學校名稱: | 國立臺灣海洋大學
系所名稱: | 食品科學系
學號: | 19932067
畢業學年度: | 100
語文別: | 中文
關鍵字: | 海藻寡醣 Marin algal oligosaccharides
| 高度硫酸化 Oversulfated
| 抗病毒 Antiviral
| 抗凝血 Anti-thrombin
| 抗血小板凝集 Anti-platelet Aggregation
| Caco-2 細胞 Caco-2 cell
全文影像連結: | | (本論文20150730對校內公開)
| 論文全文(pdf)

論文頁數: | 155
摘要: | 本研究目的在於探討馬尾藻、龍鬚菜、及石蓴熱萃液經酵素水解後區分不同聚合度的寡醣，利用氯磺酸-二甲基甲醯胺法 (Chlorsulfonic acid-N, N-dimethylformamide method, DMF)，提升其硫酸基含量，並比較所得高硫酸基含量海藻寡醣對人血漿抗凝血與抗血小板凝集和抗病毒活性及對腸道細胞株 Caco-2 吸收之影響。馬尾藻、龍鬚菜、及石蓴於 121oC 熱萃 40 min 後以 3% 纖維素酶水解 6 hr，以及 2 U/mL 海藻熱萃多醣粉末誘導 Pseudomonas vesicularis MA103 及 Aeromonas salmonicida MAEF108 所產之酵素水解 24 hr 後，利用超過濾系統區分出小於 3 kDa 分子量之寡醣，經過 Sephadex G-10 膠體過濾層析 (Gel permeation chromatography, GPC) 及高效能液相層析儀 (High performance liquid chromatography, HPLC) 可初步分離出 9 種不同聚合度的寡醣，馬尾藻Sar-Y2、Sar-Y4、及 Sar-Y6，龍鬚菜 Gra-Y2、Gra-Y4、及Gra-Y6，石蓴寡醣 Ulva-Y2、Ulva-Y4、及Ulva-Y6。經區分後之海藻寡醣利用 DMF 法於 25℃ 下反應 4 小時，結果顯示Sar-Y2、Sar-Y4、及Sar-Y6硫酸基含量從 1.1-3.7% 提升至 17.8%-29.6%，Gra-Y2、Gra-Y4、及Gra-Y6從1.3-4.4% 提升至 13.7%-30.4%，Ulva-Y2、Ulva-Y4、及Ulva-Y6從0.9%-3.4% 提升至 14.7%-26.4%，而 Heparin (69.2 U/mL) 之硫酸基含量為 27.2%。經 HPLC分析得知，與標準品及分子質量檢量線比對初步可分得可能聚合度 (Degree of polymerization, DP) 為2、4、及6之寡醣，並進一步以揮發性光散射偵測器 (Evaporative light scattering detector, ELSD) 偵測，皆只有一個明顯之波峰 ; 且高硫酸基含量海藻寡醣以 HPLC 分析其滯留時間皆會提前，推測為寡醣接上硫酸基所造成。以傅立葉轉換紅外線光譜儀 (Fourier transform infrared spectroscopy, FT-IR) 分析，可在波長數 822 cm-1 及 1,250 cm-1 處左右有明顯之硫酸基團 C-O-S 及 S=O 之伸縮振動，推測為硫酸基添加所造成之結果。在人血漿之抗凝血實驗中，於活化部分凝血活酶時間 (Activated partial thromboplastin time, APTT)，10 mg/mL Heparin 可延遲至 55 sec，而 20 mg/mL 的 Sar-Y4 DMF、 Sar-Y6 DMF、Gra-Y4 DMF、Gra-Y6 DMF、及 Ulva-Y6 DMF 可分別延遲至 66 sec、 99 sec 、55 sec、99 sec、及 77 sec，而在凝血酶原時間 (Prothrombin time, PT) 實驗中，高硫酸基含量之寡醣皆無延遲 PT 之活性，但會隨著硫酸基含量及寡醣濃度提高而降低凝血程度。於抗血小板凝集實驗中，20 mg/mL 的 Sar-Y2 DMF、Sar-Y4 DMF、Sar-Y6 DMF、Gra-Y6 DMF、與 Ulva-Y6 DMF 較相對應未添加硫酸基寡醣之凝集百分比分別降低了 12.2%、12.5%、11.7%、3.8%、與 11.6%，且皆與相對之控制組有顯著差異。而高硫酸基含量馬尾藻、龍鬚菜、及石蓴寡醣於抗日本腦炎病毒北京 1 型、登革熱病毒第 2 型、及腸病毒 71 型上，結果顯示主要的抑制效果在於抑制病毒吸附至宿主細胞上 (細胞存活率皆大於 92%)，與單純病毒感染組 (Cell + Virus) 相較下皆有顯著性差異 (*p＜0.05)。對腸道細胞株 Caco-2 細胞吸收測試之實驗上，以 HPLC 分析結果顯示，於 Transwell insert 吸收測試裝置之下層液，並未見到有寡醣被細胞吸收而穿透至下層液中，也未測得其醣量，但在上層液中，隨著作用時間 (0 min、30 min、及 90 min) 的增加，寡醣的含量則會隨之下降。
| The aim of this study is to separate oligosaccharides (OS) with different degree of polymerization (DP) from hydrolysate of Sargassum sp., Gracilaria sp., and Ulva lactuca polysaccharides (PS), and using chlorsulfonic acid- N, N-dimethylformamide (DMF) method to increase sulfate contents of these OS. The effects of their anticoagulant, anti-platelet aggregation, antiviral activities, and its adsorption performance with intestinal cell line Caco-2 are examined. Sargassum sp., Gracilaria sp., and Ulva lactuca was extracted by 121oC hot water to obtain PS, and then added 3% cellulase, 2 U/mL crude PS degrading enzymes from Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108 (induced with Sargassum sp., Gracilaria sp., and Ulva lactuca PS) for enzyme hydrolysis (24 hr), Oligosaccharides hydrolysates with molecular mass < 3 kDa were collected by ultra filtration (UF) system, then though gel permeation chromatography (GPC) and high performance liquid chromatography (HPLC) to obtain isolated OS with different DP, such as Sar-Y2, Sar-Y4, and Sar-Y6 (from Sargassum sp.), Gra-Y2, Gra-Y4, and Gra-Y6 (from Gracilaria sp.), Ulva-Y2, Ulva-Y4, and Ulva-Y6 (from Ulva lactuca). The isolated OS then through DMF method (25oC for 4 hr) to increase sulfate contents. The sulfate contents of Sar-Y2, Y4, Y6 from 1.1 %-3.7% to 17.8%-29.6%, Gra-Y2, Y4, Y6 from 1.3%-4.4% to 13.7%-30.4%, Ulva-Y2, Y4, Y6 from 0.9%-3.4% to 14.7%-26.4%, and heparin (69.2 U/mL) was 27.02%. The HPLC analysis of isolated OS compare to standard and molecular mass calibration curve, the DP of OS may be DP 2, 4, 6, and using evaporative light scattering detector (ELSD) to detected,the results show only one obvious peak. The oversulfated algal OS analysis by HPLC, its retention time are earlier than original non-oversulfated OS. Fourier transform infrared spectroscopy (FT-IR) showed that was obvious sulfate ester group signal enhancement in wave numbers 822 cm-1 and 1,250 cm-1 are C-O-S and S=O stretching vibration, resulted from higher increase of sulfate contents. In the experiment of anticoagulant activities of human plasma, activated partial thromboplastin time (APTT) analysis of Heparin (10 g/mL) was delayed to 55 sec, however, APTT of Sar-Y4 DMF, Sar-Y6 DMF, and Gra-Y4, Gra-Y6 DMF, and Ulva-Y6 DMF (20 mg/mL) were delayed to 66 sec, 99 sec, 55 sec, 99 sec, and 77 sec, respectively. The anticoagulant activities of human plasma in prothrombin time (PT) analysis were examined, oversulfated OS were no obvious delayed in PT, but there were a tendency for reduction on absorbance value while OS concentration were increased. In anti-platelet aggregation analysis, percentage of platelet aggregation of 20 mg/mL Sar-Y2 DMF, Sar-Y4 DMF, Sar-Y6 DMF, Gra-Y6 DMF, and Ulva-Y6 DMF had lower 12.2%, 12.5%, 11.7%, 3.8%, and 11.6% than original related non-oversulfated OS, respectively, and had significant difference with control. In anti-japanese encephalitis virus Beijing 1 strain, dengue virus type 2, and enterovirus type 71 experiments, the results show the inhibition activity at prevent assay (cell viability＞92%) and had significant difference with virus alone (*p＜0.05). In the adsorption performance with Caco-2 cells testing experiment, the HPLC analysis showed that the basolateral fluid in the adsorption test device (Transwell insert), did not observe the OS uptake by cells and transfer to the basolateral fluid. But in the apical fluid, as the reaction time (0 min, 30 min, and 90 min) increased, that the OS contents were decreased.
論文目次: | 目 錄
|
| 目錄            i
| 表目錄            vii
| 圖目錄            viii
| 附錄目錄    xiii
| 中文摘要    xiv
| 英文摘要 (Abstract)    xvi
| 壹、前言        1
| 貳、文獻整理        3
| 一、海藻之來源與應用        3
| 1-1. 海藻簡介        3
| 1-2. 馬尾藻        4
| 1-3. 龍鬚菜        4
| 1-4. 石蓴        5
| 二、海藻寡醣        5
| 2-1. 海藻寡醣之萃取方法        5
| 2-2. 海藻寡醣之生理活性        6
| 2-2-1. 抗病毒        6
| 2-2-2. 抗氧化        7
| 2-2-3. 抗凝血及抗血小板凝集活性        8
| 2-2-4. 護肝作用        9
| 2-2-5. 益菌性        10
| 2-2-6. 調節免疫活性與抗腫瘤        10
| 2-2-7. 抑制血管收縮素轉化酵素 (Angiotesin
| converting enzyme)        11
| 三、海藻寡醣高度硫酸化方法        11
| 四、海藻寡醣組成成分之鑑定        13
| 4-1. 高效能液相層析法 (High-performance liquid
| chromatography, HPLC)        13
| 4-2. 傅立葉轉換紅外線光譜儀 (Fourier-transform
| Infrared Spectroscopy, FTIR)        13
| 五、血液凝血機制        14
| 5-1. 內在途徑        15
| 5-2. 外在途徑        16
| 5-3. 最終共同路徑        16
| 5-4. 凝血酶的正回饋機制        17
| 5-5. 凝血作用的測試        17
| 5-6. 硫酸化多醣及寡糖對凝血的影響        18
| 5-7. 抗凝血系統        18
| 5-7-1. 抗凝血蛋白酶 III (Antithrombin III)        18
| 5-7-2. 蛋白質 C 與其輔因子蛋白質 S        18
| 5-7-3. 組織因子路徑抑制劑 (Tissue factor pathway
| inhibitor, TFPI)        19
| 5-8. 纖維蛋白溶解系統 (Fibrinolytic or thrombolytic
| system)        19
| 六、血小板        19
| 6-1. 血小板構造及其生理功能        19
| 6-2. 血小板凝集作用 (Aggregation)        20
| 七、日本腦炎病毒        21
| 7-1. 封套蛋白結構特性及作用機制        21
| 7-2. 感染細胞機制        22
| 八、登革熱病毒        22
| 8-1. 病毒特性及傳染模式        22
| 8-2. 感染細胞機制        23
| 九、    腸病毒        24
| 9-1. 病毒特性及增殖周期        24
| 9-2. 感染途徑及入侵途徑        25
| 十、腸道細胞株 Caco-2        25
| 10-1. Caco-2 細胞簡介        25
| 10-2. Caco-2 細胞之吸收模式        26
| 10-2-1. 藥物吸收模式        26
| 10-2-2. 醣類吸收模式        27
| 参、實驗設計        28
| 肆、材料方法        29
| 一、實驗材料        29
| 1-1. 原料        29
| 1-2. 試驗菌株        29
| 1-3. 試驗細胞株        29
| 1-4. 試驗病毒株        29
| 1-5. 試驗藥品        29
| 1-6. 儀器設備        31
| 1-7. 培養基組成        33
| 1-7-1. P. vesicularis MA 103 多醣降解酵素誘導
| 培養基    33
| 1-7-2. A. salmonicida MAEF108多醣降解酵素誘導
| 培養基    33
| 1-8. 細胞培養基        34
| 1-9. 海藻誘導酵素反應基質        34
| 1-10. DNS 溶液        35
| 二、實驗方法        35
| 2-1. 菌株之保存與活化        35
| 2-1-1. 菌株之保存        35
| 2-1-2. 菌株之活化        35
| 2-2. 海藻之一般成分分析        35
| 2-2-1. 水分含量        35
| 2-2-2. 灰分含量        36
| 2-2-3. 粗蛋白含量        36
| 2-2-4. 粗脂肪含量        36
| 2-2-5. 粗纖維含量        37
| 2-3.    P. vesicularis MA103 或 A. salmonicida MAEF108 經
| 個別海藻熱萃多醣誘導所產之水解酵素與其活性測定        37
| 2-3-1. 個別海藻熱萃多醣之製備        37
| 2-3-2. 菌株活化與誘導        37
| 2-3-3. 個別海藻熱萃多醣誘導酵素之生產        38
| 2-3-4. 個別海藻熱萃多醣誘導酵素的活性測定        38
| 2-4. 個別海藻寡醣之分離與純化        39
| 2-4-1. 海藻寡醣水解物製備        39
| 2-4-2. 超過濾 (Ultra filtration, UF)        39
| 2-4-3.    GPC 膠體過濾層析        39
| 2-5. 高度硫酸化        39
| 2-5-1. 氯磺酸–二甲基甲醯胺法        39
| 2-6. 海藻寡醣與高硫酸基含量海藻寡醣之成分分析        40
| 2-6-1. 還原糖含量        40
| 2-6-2. 總醣含量        40
| 2-6-3. 硫酸基含量測定        40
| 2-6-4. 高效能液相層析法 (High-performance liquid
| chromatography, HPLC)        41
| 2-6-5. 傅立葉轉換紅外線光譜法 (Fourier transform
| infrared spectroscopy, FT-IR)        42
| 2-7. 抗凝血測定        42
| 2-7-1. 活化部份凝血活酶時間 (Activited partial
| thromboplastin time, APTT)        42
| 2-7-2. 凝血酶原時間 (Prothrombin time, PT)        42
| 2-8. 抗血小板凝集 (Platelet aggregation) 生理活性        43
| 2-8-1. 富含血小板血漿之製備        43
| 2-8-2.    血小板凝集測定 (Platelet aggregation
| measurement)        43
| 2-9. 評估高硫酸基含量海藻寡醣抗病毒能力        44
| 2-9-1. 細胞株培養方法及繼代方法        44
| 2-9-2. 細胞毒性試驗 (MTT assay)        44
| 2-9-3. 抗日本腦炎病毒、登革熱病毒、及腸病毒試驗        45
| 2-9-3-1. 抑制病毒對宿主細胞吸附反應及抑制病毒
| 繁殖反應        45
| 2-10. 海藻寡醣腸道細胞吸收試驗        46
| 2-10-1.    Caco-2 細胞株之活化        46
| 2-10-2.    Caco-2 細胞株培養方法及繼代方法        46
| 2-10-3.    細胞數計數        47
| 2-10-4.    細胞之冷凍保存        47
| 2-10-5.    Caco-2 細胞存活率分析 (MTT assay)        47
| 2-10-5-1.    不同 Caco-2 細胞接種數之細胞
| 存活率測定        48
| 2-10-5-2.    高硫酸基含量海藻寡醣對Caco-2 細胞
| 之毒性試驗        48
| 2-10-6.    Transwell insert 培養法        48
| 2-10-7.    Caco-2 細胞於吸收裝置其單層膜完整性測試        48
| 2-10-8.    高硫酸基含量海藻寡醣於腸道細胞吸收率測定        49
| 2-11. 統計分析        49
| 伍、結果與討論        50
| 陸、結論        66
| 柒、參考文獻        68
|
參考文獻: | 柒、參考文獻
|
| 王彥容。2007。重緣葉馬尾藻多醣類及其奈米粒抗細胞增生效果之探討。國立臺灣海洋大學食品科學系碩士學位論文。基隆。臺灣。
| 王順春、方積年。1999。香菇多醣硫酸化衍生物的製備及其結構分析。生物化學與生物物理學報。31 (5): 594-597。
| 田庚元、李壽桐、宋麥麗。1995。牛膝多醣硫酸酯的合成及其抗病毒活性。藥學學報。30 (2): 107-111。
| 史寶軍、聶小華、許泓渝、陶文沂。2003。灰樹花多醣硫酸酯的製備及其抗腫瘤活性。中國醫藥工業雜誌。34 (8): 383-385。
| 吳全耀。1998。巧味芽 (龍鬚菜) 的養殖管理、綠化及一般成分。漁業推廣。144：21-26。
| 吳全耀。2001。巧味芽 (龍鬚菜) 為保健食品的利用。漁業推廣。173：46-52。
| 吳紹祺。1999。海洋細菌所產 agarases 生產條件與海藻多醣經 agarase 分解所得寡醣類組成之探討。國立臺灣海洋大學食品科學系碩士學位論文。基隆。臺灣。
| 吳紹祺。2005。海藻多醣洋菜酶水解物及其發酵產物之生理活性研究。國立臺灣海洋大學食品科學系博士學位論文。基隆。臺灣。
| 吳德培譯。2009。止血與血栓形成。生物化學。pp. 606-616。合記圖書出版社。臺北，臺灣。
| 何敏夫。2002。血液學 (3rd ed.)。合記圖書出版社。臺北。
| 臺灣。
| 李冠青。2008。食品多醣、甲基化衍生物及降解物之抗氧化活性及對腸腺癌細胞之抑制性。靜宜大學食品營養學系碩士學位論文。臺中。臺灣。
| 李樵。2009。提升青海菜寡醣硫酸基含量及其產物生理活性之探討。
| 國立臺灣海洋大學食品科學系碩士學位論文。基隆。臺灣。
| 沈贇、尹鴻萍、王旻。2005。螺旋藻中性多醣硫酸化及性質研究。藥物生物技術。1 (6): 22-25。
| 林季磊。2011。海藻寡醣經分離與提高硫酸基含量後之生理活性探討。國立臺灣海洋大學碩士學位論文。基隆。臺灣。
| 林岑穎、施姵岑、卡伯特 (Bartosz Kazlowski)、馮家輝、柯孟宏、江孟燦、潘崇良。2008。新洋菜寡醣之抗凝血活性。臺灣農業化學會年會，2008 年6 月30 日，臺北。p. 91，(B14)。
| 林啟弘。2003。紅豆水煮過程中子葉組織理化特性變化之研究。國立屏東科技大學碩士學位論文。屏東。臺灣。
| 邱雅凰。2007。海藻萃取物抑制日本腦炎病毒感染及免疫功能調節之
| 研究。國立台灣海洋大學食品科學系碩士學位論文。基隆。台灣。
| 胡幼圃。2000。美國藥學科學家會議報告。科學發展月刊。29:
| 283-285。
| 洪啟芳。2003。硫化程度對一些多醣及寡醣之抗凝血性質之探討。大同大學生物工程研究所碩士學位論文。臺北。臺灣。
| 施珊汶。2006。海帶多醣水解液乳酸發酵產物之生理活性探討。國立
| 臺灣海洋大學食品科學系碩士學位論文，基隆。臺灣。
| 施姵岑、林岑穎、廖友豪、王瓊誼、卡伯特 (Bartosz Kazłowski)、江孟燦、潘崇良。2008。海菜寡醣抗凝血活性之探討。臺灣農業化學會年會，2008 年6 月30 日，臺北。p. 88，(B11)。
| 施姵岑。2009。石蓴寡醣之生產與其生理活性之探討。國立臺灣海洋
| 大學食品科學系碩士學位論文。基隆。臺灣。
| 高慶煌。2002。以熱分析法測定基丁質類物質之乙醯化程度。國立臺
| 灣海洋大學食品科學系碩士學位論文，基隆。臺灣。
| 陸王行、何子萬。1989。有機分子光譜分析。中央圖書出版。13-50，臺北。臺灣。
| 許雅捷。2004。幾丁聚醣對於細胞在附著及生長之影響。國立臺灣海洋大學食品科學系碩士學位論文。基隆，臺灣。
| 陳長安。2006。抗凝血劑。常用藥物治療手冊。pp. 892-902。全國藥品年鑑雜誌社。臺北，臺灣。
| 康新楷。2007。Aeromonas salmonicida MAEF108 所產MAEF108 Agarse II之純化與特性研究暨經Agarase AS-II 水解所得藻類寡醣之生理活性探討。國立臺灣海洋大學食品科學系碩士學位論文
| 。基隆。臺灣。
| 郭馨鎂。 2006。海藻寡糖對雙叉桿菌之助生性及在發酵乳應用之研
| 究。國立臺灣大學食品科技研究所碩士學位論文。臺北。臺灣。
| 傅子恆。2009。新洋菜寡醣之硫酸基含量提升對抗氧化與抗凝血活性之影響。國立臺灣海洋大學食品科學系碩士學位論文。基隆。臺灣。
| 馮家輝、鍾佳華、李欣翰、卡伯特 (Bartosz Kazlowski)、潘崇良。2007。酸和agarases組合降解龍鬚菜多醣液所得寡醣之組成分析，臺灣  食品科學技術學會年會，2007年12 月07 日，彰化。B-57。
| 黃淑芳。1989。認識藻類。臺灣省立博物館出版。臺北市。 pp. 1-54。
| 黃淑芳。2000。臺灣東北角海藻圖錄。臺灣博物館。台北。 pp. 23-25。
| 黃霨禎。2005。龍鬚菜寡糖水解物之製備及其抗氧化與抗至突變性之探討與對雙叉桿菌生長影響。國立臺灣海洋大學食品科學系碩士學位論文。基隆。臺灣。
| 劉綉婷、馮家輝、許志維、廖友豪、李樵、潘崇良。2008。海藻寡醣熱萃水解液之抗氧化活性評估。臺灣保健食品學會年會，2008 年 2 月 22 日，臺北。p. 67，(PA-035)。
| 潘淑芬編譯。2003。止血反應與血栓形成。醫學生物化學修訂版。pp.
| 55-67。藝軒圖書出版社。新北市，臺灣。
| 潘震澤、楊志剛、高毓儒、黃娟娟、袁宗凡編譯。2006。心血管生理。
| 人體生理學。pp. 449-466。合記圖書出版社。臺北，臺灣。
| 蔡麗雯。2009。石蓴多醣粗萃物與鹿角菜膠液治腸病毒七十一型感染之研究。國立臺灣海洋大學食品科學系碩士學位論文。基隆。臺灣。
| Amarasekara, A. S., G. Opoku, X. Qiu, and V. Doctor. 2007. Effect of oversulfation on the chemical and biological properties of chondroitin-6-sulfate. Carbohydrate Polymers 68:116-121.
| AOAC. 1997. Offical Methods of Analysis. 16th edition. Association of Official Analytical Chemists. Washington, DC, USA.
| Athukorala, Y., W. K. Jung, T. Vasanthan, and Y. J. Jeon. 2006. An anticoagulative polysaccharide from an enzymatic hydrolysate of Ecklonia cava. Carbohydrate Polymers 66: 184-191.
| Balimane, P.V., and S. Chong. 2005. Cell culture-based models for intestinal permeability: A critique. Drug Discovery Today 10: 335-343.
| Braun, A., S. H.merle, K. Suda, B. Rothen-Rutishauser, M. G. Thert, S. D. Krer, and H. Wunderli-Allenspach. 2000. Cell cultures as tools in biopharmacy. European Journal of Pharmaceutical Sciences 11: 51-60.
| Buenz, E. J., and C. L. Howe. 2006. Picornaviruses and cell death.
| Trends in Microbiology 14: 28-36.
| Calvete, J. J. 1994. Clues for understanding the structure and function of
| a prototypic human integrin: The platelet glycoprotein IIb/IIIa
| complex.  Journal of Thrombosis and Haemostasis 72: 1-15.
| Carlucci, M. J., C. A. Pujol, M. Ciancia, M. D. Noseda, M. C. Matulewicz, E. B. Damonte, and A. S. Cerezo. 1997. Antiherpetic and anticoagulant properties of carrageenans from the red seaweed
| Gigartina skottsbergii and their cyclized derivatives: Correlation between structure and biological activity. International Journal of Biological Macromolecules 20: 97-105.
| Chan, K. C., P. P. Lou, and J. L. Hargrove. 1993. High
| casein-lactalbumin diet accelerates blood coagulation in rats. The
| Journal of Nutrition 123: 1010-1016.
| Chen, Y., T. Maguire, R. E. Hileman, J. R. Fromm, J. D. Esko, R. J. Linhardt, and R. M. Marks. 1997. Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate. Nature Medicine 3: 866-871.
| Chen, H., X. Yan, P. Zhu, and J. Lin.  2006.  Antioxidant activity and hepatoprotective potential of agaro-oligosaccharides in vitro and in vivo.  Nutrition Journal 5: 31-43.
| Cory, A. H., T. C. Owen, J. A. Barltrop, and J. G. Cory.  1991.  Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture.  Cancer Communication 3: 207-212.
| Cumashi, A., N. A. Ushakova, M. E. Preobrazhenskaya, A. D'Incecco, A. Piccoli, L. Totani, N. Tinari, G. E. Morozevich, A. E. Berman, M. I. Bilan, A. I. Usov, N. E. Ustyuzhanina, A. A. Grachev, C. J. Sanderson, M. Kelly, G. A. Rabinovich, S. Iacobelli, and N. E. Nifantiev. 2007. A comparative study of the anti-inflammatory, anticoagulant,  ntiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17: 541-552.
| Dace, R., E. McBride, K. Brooks, J. Gander, M. Buszko, and V. M. Doctor. 1997. Comparison of the anticoagulant action of sulfated and phosphorylated polysaccharides. Thrombosis Research 87: 113-121.
| De    Palma, A. M., L. Vliegen, E. De Clercq, and J. Neyts. 2008.
| Selective inhibitors of picornavirus replication. Medicinal Research
| Reviews 28: 823-884.
| Doctor, V. M., D. Lewis, M. Coleman, M. T. Kemp, E. Marbley, and V. Sauls. 1991. Anticoagulant properties of semisynthetic polysaccharide sulfates.  Thrombosis Research 64: 413-425.
| Dodgson, K. S. 1961. Determination of inorganic sulfate in studies on the enzymatic and non-enzymatic hydrolysis of carbohydrate and other sulfate esters. The Journal of Biochemistry 78: 312-319.
| D'Souza, S. E., M. H. Ginsberg, T. A. Burke, S. C. Lam, and E. F.
| Plow. 1988. Localization of an Arg-Gly-Asp recognition site within
| an integrin adhesion receptor. Science 7: 91-93.
| Dubois, M., K. A. Gilles, J. K. Hamilton, P. A.Rebers, and F. Smith.
| 1956.  Colormetric method for determination of sugars and related
| substances.  Analytical Chemistry 28: 350-356.
| Durate, M. E. R., M. A. Cardoso, M. Noseda, and A. S. Cerezo.  2001.  Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum.  Carbohydrate Research 333: 281-293.
| Gan, L. S. L., and D. R. Thakker. 1997. Applications of the Caco-2 model in the design and development of orally active drugs: elucidation of biochemical and physical barriers posed by the intestinal epithelium. Advanced Drug Delivery Reviews 23: 77-98.
| Gnoth, M. J., S. Rudloff, C. Kunz., and R. K. H. Kinne. 2001. Investigations of the in vitro transport of human milk oligosaccharides by a Caco-2 monolayer using a novel high performance liquid chromatography-mass spectrometry technique.  The Journal of Biological Chemistry 276: 34363-34370.
| Gnoth, M. J., S. Rudloff, C. Kunz, and R. K. H. Kinne, R. 2002. Studies on the intestinal transport of human milk oligosaccharides (HMO) using Caco-2 cells. Food Research International 35: 145-49.
| Gubler, D. J. 1998. Dengue and dengue hemorrhagic fever. Clinical
| Microbiology Reviews 11: 480-496.
| Hartwig, J., and J. Italiano. 2003. The birth of the platelet. Journal of
| Thrombosis and Haemostasis 7: 1580-1586.
| Hazelton, G. A., J. J. Hjelle, R. L. Dills, and C. D. Klaassen.  1985.  A radiometric method for the measurement of 3’-phosphoadenosine -5’-phosphosulfate in rat and mouse liver.  Drug Metabolism and Disposition: The Biological Fate of Chemicals 13: 30-34.
| Heo, S. J., and Y. J. Jeon.  2009.  Protective effect of fucoxanthin isolated from Sargassum siliquastrum on UV-B induced cell damage.  Journal of Photochemistry and Photobiology B, Biology  95: 101-107.
| Hidalgo, I. J., T. J. Raub, and R.T. Borchardt. 1989. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96: 736-749.
| Hillgren, K. M., A. Kato, and R.T. Borchardt. 1995. In vitro systems for studying intestinal drug absorption. Medicinal Research Reviews 15: 83-109.
| Hu, B., Q. Gong, Y. Wang, Y. Ma, J. Li, and W. Yu.  2006.  Prebiotic effects of neoagaro-oligosaccharides prepared by enzymatic hydrolysis of agarose. Anaerobe 12: 260-266.
| Hung, H. C., C. P. Tseng, J. M. Yang, Y. W. Ju, S. N. Tseng, Y. F.  Chen, Y. S. Chao, H. P. Hsieh, S. R. Shih, and J. T. Hsu. 2009.
| Aurintricarboxylic acid inhibits influenza virus neuraminidase.
| Antiviral Research 81(2): 123-131.
| Hunter, J., M. A. Jepson, T. Tsuruo, N. L. Simmons, B. H. Hirst. 1993. Functional expression of P-glycoprotein in apical membranes of human intestinal Caco-2 cells. Kinetics of vinblastine secretion and interaction with modulators. The Journal of Bioligical Chemistry 268(20): 14991-14997.
| Jennings, L. K., and D. R. Phillips. 1982. Purification of glycoproteins
| IIb and III from human platelet plasma membranes and
| characterization  of a calcium-dependent glycoprotein IIb-III
| complex. The Journal of Biological Chemistry 257: 10458-10466.
| Kato, A., K.Yamamoto, and N. Aoki. 1992. Classification of
| Glanzmann's thrombasthenia based on the intracellular transport
| pathway of GPIIb-IIIa. Journal of Thrombosis and Haemostasis 68:
| 615-616.
| Kawano, H., V. Rostapshov, L. Rosen, and C. J. Lai. 1993. Genetic determinants of dengue type 4 virus neurovirulence for mice. Journal of Virology 67: 6567-6575.
| Kazlowski, B. 2010. Studies on the Isolation, Identification and Applications of Low-Degree-Polymerization Algal Saccharides and Mechanism of Agaro-Polysaccharide Degradations. Ph.D. Dissertation, Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.
| Kazlowski, B., Y. H. Chu, K. Kazłowska, C. L. Pan, and C. J. Wu.
| 2012. Prevention of Japanese encephalitis virus infections by
| low-degree-polymerisationsulfated saccharides from Gracilaria sp.and Monostroma nitidum. Food Chemistry 133: 866-874.
| Kazlowski, B., and C. L. Pan.  2008.  Separation and quantification of Monostroma-oligosaccharide from Monostroma nitidum and neoagaro-oligosaccharide from Gracilaria tenuistipitata products obtained via digestion of Aeromonas salmonicida MAEF108  -agarases by preparative HPLC and NMR analysis.  June 30th, 2008.  C42, p. 125.  Agricultural Chemical Society of Taiwan, Taipei, Taiwan, R. O. C.
| Kazlowski, B., Y.-T. Ko, and C.-L. Pan. 2007 Comparison of two neoagaro-oligosaccharide production systems in the quantification of their oligomer products generated from agarose by -agarase digestion.  December 7th, 2007.  B-56.  Taiwan Association for Food Science and Technology, Changhua, Taiwan, R. O. C.
| Kazlowski, B., Y. H. Chiu, C. L. Pan, and C. J. Wu. 2008a. Antiviral activity of sulfated oligosaccharides from algae Monostroma nitidum and Gracilaria tenuistipitata obtained by digestion of MAEF108 -agarases from Aeromonas salmonicida.  June 30th, 2008.  A49, p. 69.  Agricultural Chemical Society of Taiwan, Taipei, Taiwan, R. O. C.
| Kazlowski, B., K. Kazłowska, C. L. Pan, and G. J. Tsai.  2008b.  Stimulation on nitric oxide production in RAW 264.7 macrophages by defined chain lengths oligosaccharides derived from Gracilaria tenuistipitata and Monostroma nitidum. May 8th to 9th, 2008.  BT-03, p. 270.  International Conference of Advanced Research on Marine Bioresources, Keelung, Taiwan, R. O. C.
| Kazlowski, B., C. L. Pan, and Y. T. Ko.  2008c.  Separation and quantiﬁcation of neoagaro- and agaro-oligosaccharide products generated from agarose digestion by -agarase and HCl in liquid chromatography systems. Carbohydrate Research 343: 2443–2450.
| Ke, Y.Y.,Y. C. Chen, and T. H. Lin. 2006. Structure of the virus capsid
| protein VP1 of enterovirus 71 predicted by some homology
| modeling and molecular docking studies. Journal of Computational
| Chemistry 27: 1556-1570.
| Kieffer, N. 1996. Adhesive platelet glycoproteins and platelet function.
| In: Adhesion Receptors As Therapeutic Targets. Ed. by Horton,
| M. A., CRC Press, Boca Raton, FL, U.S.A., PP. 139-169.
| Knutsen, S. H., and H. Grasdalen. 1992. Analysis of carrageenans by enzymic degradation, gel filtration and 1H NMR spectroscopy. Carbohydrate Polymers 19: 199-210.
| Koyanagi, S., N. Tanigawa, H. Nakagawa, S. Soeda, and H. Shimeno.  2003.  Oversulfation of fucoidan enhances it anti-angiogenic and antitumor activities.  Biochemical Pharmacology  65: 173-179.
| Laitinen, L., H. Kangas, A. M. Kaukonen, K. Hakala, T. Kotiaho, R. Kostiainen, and J. Hirvonen. 2003. N-in-one permeability studies of heterogeneous sets of compounds across Caco-2 cell monolayers.
| Pharmaceutical Research 20: 187-197.
| Lee, J. B., S. Koizumi, K. Hayashi, and T. Hayashi. 2010. Structure of rhamnan sulfate from green alga Monostroma nitidum and its anti-herpetic effect. Carbohydrate Polymers 385: 199-203.
| Li, C., H. T. Wang, S. R. Shih, T. C. Chen, and M. L. Li. 2007. The
| efficacy of viral capsid inhibitors in human enterovirus infection and
| associated diseases. Current Medicinal Chemistry 14: 847-856.
| Li, C. C., M. Y. Yang, R. F. Chen, T. Y. Lin, K. C. Tsao, H. C
| Ning, H. C. Liu, S. F. Lin, W. T. Yeh, and Y. T. Chu. 2002.
| Clinical manifestations and laboratory assessment in an enterovirus
| 71 outbreak in southern Taiwan. Scandinavian Journal of Infectious
| Diseases 34: 104-109.
| Lin, S. R., S. C. Hsieh., Y. Y. Yueh., T. H. Lin., D. Y. Chao., W. J. Chen., C. C. King, and W. K. Wang. 2004. Study of sequence variation of dengue type 3 virus in naturally infected mosquitoes and human hosts: implications for transmission and evolution. Journal of Virology 78: 12717-12721.
| Liu, H., S. S. Chiou, and W. J. Chen. 2004. Differential binding efficiency between the envelope protein of Japanese encephalitis virus variants and heparan sulfate on the cell surface. Journal of Medical Virology 72(4): 618-624.
| Marianneau, P., M. Flamand, V. Deubel, and P. Despres. 1998. Apoptotic cell death in response to dengue virus infection: The pathogenesis of dengue haemorrhagic fever revisited. Journal of Clinical Virology 10: 113-119.
| Marks, R. M., H. Lu., R. Sundaresan, T. Toida, A. Suzuki, T. Imanari, M. J. Hernaiz, and R. J. Linhardt. 2001. Probing the interaction of dengue virus envelope protein with heparin: assessment of glycosaminoglycan-derived inhibitors. Journal of Medicinal Chemistry 44(13): 2178-2187.
| Matsubara, K., Y. Mastsuura, A. Bacic, M.-L. Liao, K. Hori, and K. Miyazawa. 2001. Anticoagulant properties of a sulfate galactan preparation from a marin green alga, Codium cylindricum.  Biomacromolecules  28: 395-399.
| McMinn, P. C. 2002. An overview of the evolution of enterovirus 71
| and its clinical and public health significance. FEMS Microbiology Reviews 26: 91-107.
| Miller, G. L.  1959.  Use of dinitrosalicylic acid reagent for reagent for deterimination of reduceing sugars. Analytical Chemistry 31: 426-428.
| Nash, G. F., L. F. Turner, M. F. Scully, and A. K. Kakker. 2002.
| Platelets and cancer. The Lancet Oncology 3: 425-430.
| Naumann, P. 2000. Infrared spectroscopy in Microbiology. Encyclopedia of Analytical Chemistry. Ed. by Meyers, R. A.,
| John Wiley and Sons, Inc, Chichester, U.K., PP. 102-131.
| Nishion, T., and T. Nagumo. 1991. The sulfate-content dependence of the anticoagulant activity of a fucan sulfate from the brown seaweed  Ecklonia Kurome. Carbohydrate Research  214: 193-197.
| Nurden, A. T. 1999. Inherited abnormalities of platelets. Journal of Thrombosis and Haemostasis 82: 468-480.
| Ohta, Y., Y. Nogi, M. Miyazaki, Z. Li, Y. Hayada, S. Ito, and K. Horikoshi. 2004. Enzymatic properties and nucleotide and amino acid sequences of a thermostable β-agarase from the novel marine isolate, JAMB-A94.  Bioscience, Biotechnology, and Biochemistry 68: 1073-1081.
| Peterson, M. D., and M. S. Mooseker. 1992. Characterization of the enterocyte-like brush border cytoskeieton of the C2BBe clones of the human intestinal cell line, Caco-2. Journal of Cell Science 102: 581-600.
| Phillips, D. R., L. Nannizzi-Alaimo, and K. S. Prasad. 2001. Beta3
| tyrosine phosphorylation in alphaIIbbeta3 (platelet membrane GP
| IIb-IIIa) outside-in integrin signaling. Journal of Thrombosis and
| Haemostasis 86: 246-258.
| Plumb, J. A., R. Milroy, and S. B. Kaye. 1989 Effects of the pH- dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- Tetrazolium bromide formazan absorption on chemosensitivity determined by a novel tetrazolium-based assay. Cancer Research 49: 4435-4440.
| Qiu, X., A. Amarasekara, and V. M. Doctor. 2006. Effect of oversulfationon the chemical and biological properties of fucoidan.  Carbohydrate Polymers 63: 224–228.
| Randolph, V. B., G. Winkler, and V. Stollar. 1990. Acidotropic amines
| inhibit proteolytic processing of flavivirus prM protein. Virology
| 174: 450-458.
| Rotbart, H. A. 1995. Enteroviral infections of the central nervous system. Clinical Infectious Diseases 20: 971-981.
| Rothman, A. L. 2004. Dengue: Defining protective versus pathologic
| immunity. Journal of Clinical Investigation 113: 946-951.
| Saha, S., M. H. Navid, S. S. Bandyopadhyay, and P. Schnitzler. 2012. Sulfated polysaccharides from Laminaria angustata: Structural features and in vitro antiviral activities. Carbohydrate Polymers
| 83: 123-130.
| SAS (Statistical Analytical System). 1999. SAS User's Guide: Basic Statistical Analysis. SAS Institute Inc., Cary, NC, U.S.A.
| Saunders, M. 2009. Transplacental transport of nanomaterials. WIREs Nanomedicine and Nanobiotechnology 1: 671-684.
| Schaeffer, D. J., and Krylov, V. S.  2000.  Anti-HIV activity of
| Extracts and compounds from algae and cyanobacteria.
| Ecotoxicology and Environmental Safety 45: 208-227.
| Schultz, C. P., K. Z. Liu, P. D. Kerr, and H. H. Mantsch. 1998. In situ  infrared histopathology of keratinization in human
| oral/orophharyngeal squamous cell carcinoma. Oncology Research
| 10: 277-286.
| Schultz, C. P., K. Z. Liu, E. A. Salamon, K. T. Reise, and H. H. Mantsch. 1999. Application of FT-IR microspectroscopy in diagnosing thyroid neoplasms. The Journal of Molecular Structure 481: 369-377.
| Singh, T. 1992. Agar and Agar Production. Infofish, Kuala Lumpur. Vol. 1, pp. 1-25.
| Soeda, S., S. Sakaguchi, H. Shimeno, and A. Nagamatsu. 1992.
| Fibrinolytic and anticoagulant activities of highly sulfated fucoidan.
| Biochemical Pharmacology 43: 1853-1858.
| Soeda, S., T. Kozako, K. Iwata, and H. Shimeno. 2000.  Oversulfated fucoidan inhibits the basic fibroblast growth factor-induced tube formation by human umbilical vein endothelial cells: Its possible mechanism of action. Biochimica et Biophysica Acta 1497: 127-134.
| Solomon, T. 2003. Recent advances in Japanese encephalitis. Journal of
| Neurovirology 9: 274-283.
| Su, C. M., C. L. Liao, Y. L. Lee, and Y. L. Lin. 2001. Highly sulfated forms of heparin sulfate are involved in japanese encephalitis virus infection. Virology 286 (1): 206-215.
| Sugano, Y., I. Terada, M. Arita, M. Noma, and T. Matsumoto.  1993.  Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107.  Applied and Environmental Microbiology 59: 1549-1554.
| Talarico, L. B., R. G. M. Zibetti, P. C. S. Faria, L. A. Scolaro, M.
| E. R. Duarte, M. D. Noseda, C. A. Pujol, and E. B. Damonte. 2004.  Anti-herpes simplex virus activity of sulfated galactans from
| the red seaweeds Gymnogongrus griffithsiae and Cryptonemia
| crenulata.  International Journal of Biological Macromolecules
| 34: 63-71.
| Tavelin, S., V. Milovic, G. Ocklind, S. Olsson and P. Artursson. 1999. A conditionally immortalized epithelial cell line for studies of intestinal drug transport. Journal of Pharmacology and Experimental Therapeutics 290: 1212.
| Tavelin, S., J. Taipalensuu, L. S. Erberg, R. Morrison, S. Chong, and P. Artursson. 2003. Prediction of the oral absorption of low-permeability drugs using small intestine-like 2/4/A1 cell monolayers. Pharmaceutical Research 20: 397-405.
| Terho, T. T., and K. Hartiala. 1971. Method for determination of the sulfate content of glycosaminoglycans. Analytical Biochemistry      41: 471-476.
| Vargas, F. 1988. Preparation and quantification of 3’-phosphoadenosine 5’-phospho[35S]sulfate with high specific activity. Analytical Biochemistry 172: 82-88.
| Vincent, M. L., R. M. Russell, and V. Sasak. 1985. Folic acid uptake characteristics of a human colon carcinoma cell line, Caco-2. A newly-described cellular model for small intestinal epithelium. Human Nutrition: Clinical Nutrition 39: 355-360.
| Weerachayaphorn J., and A. M. Pajor. 2008. Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2. Biochimica et Biophysica Acta (BBA) - Biomembranes 1778 (4): 1051-1059.
| Wong, K. P., B. Y. Khoo, and K. H. Sit. 1990. Biosynthesis of PAPS in vitro by human liver: Measurement by two independent assay procedures. Biochemical Pharmacology 47: 63-69.
| Wu, S. J., M. W. Chun, K. H. Shin, T. Toida, Y. Park, R. J. Linhardt, and Y. S. Kim. 1998. Chemical sulfonation and anticoagulant activity of acharan sulfate. Thrombosis Research 92: 273–281.
| Wu, S. C., C. W. Lin, S. C. Lee, and W. C. Lian. 2003. Phenotypic and
| genotypic characterization of the neurovirulence and
| neuroinvasiveness of a large-plaque attenuated Japanese encephalitis
| virus isolate. Microbes and Infection 5: 475-480.
| Yeh, S. T., C. S. Lee, and J. C. Chen. 2006. Administration of hot-water extract of brown seaweed Sargassum duplicatum via immersion and injection enhances the immune resistance of white shrimp Litopenaeus vannamei. Fish and Shellfish Immunology 20: 332-345.
| Yuan, H., J. Song, X. Li, N. Li, and J. Dai. 2006. Immunomodulation and antitumor activity of -carrageenan oligosaccharides. Cancer Letter  243: 228–234.
| Yuan, H., W. Zhang, X. Li, X. Lü, N. Li, X. Gao, and J. Song. 2005.  Preparation and in vitro antioxidant activity of -carrageenan oligosaccharides and their oversulfated, acetylated, and phosphorylated derivatives. Carbohydrate Research 340: 685–692.
| Yusof, R., S. Clum, M. Wetzel, H. M. Murthy, and R. Padmanabhan.
| 2000. Purified NS2B/NS3 serine protease of dengue virus type 2
| exhibits cofactor NS2B dependence for cleavage of substrates with
| dibasic amino acids in vitro. Journal of Biological Chemistry 275: 9963-9969.
| Zhang, Y. H., K. H. Chung, C. K. Ryu, M. H. Ko, M. K. Lee, and Y.
| P.Yun. 2001. Antiplatelet effects of 2-Chloro-3-(4-acetophenyl-amino-1,4-naphthoquinone (NQ301): A possible mechanism through
| inhibition of intracellular Ca2+ mobilization. Biological and
| Pharmaceutical Bulletin 24: 618-622.
| Zhang, Z., Q. Zhang, J. Wang, H. Song, H. Zhang, and X. Niu. 2010.
| Regioselective syntheses of sulfated porphyrans from Porphyra
| haitanensis and their antioxidant and anticoagulant activities in vitro. Carbohydrate Polymers 79: 1124-1129.
| Zhou, J., Z. Wang, S. Sun, M. Liu, and H. Zhang. 2001. A rapid method for detecting conformational changes during differetiation and apoptosis of HL60 cells by Fourier-transform infrared spectroscopy. Biotechnological Application of Biochemistry 33: 127-132.
| Zhu, Z., Q. Zhang, L. Chen, S. Ren, P. Xu, Y. Tang, and D. Luo. 2010. Higher specificity of the activity of low molecular weight fucoidan for thrombin-induced platelet aggregation. Thrombosis Research 125: 419-426.
|
|
|
|
|
|
|

|

預覽及輸出檢索結果

記錄

欄位

| 本頁 1 筆
| 記錄 4 至 50
| 選擇之記錄

| 標題
| 全部欄位
| selected fields new window
| APA Style
| Chicago Style
| MLA Style
| CNS-13611 Style
| EndNote Style

e-mail 地址 | : |
e-mail 標題 | : |

| | |

系統規劃：海洋大學圖書館
系統製作：飛資得資訊有限公司

2012 年 11 月 19 日 8:52