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論文名稱: | Pseudomonas vesicularis MA103 菌株所產 agarases PV-1 與 PV-2 之純化與性質探討
| Sudies on Purification and Characterization of Agarases PV-1 and PV-2 from Pseudomonas vesicularis MA103
研究生: | 吳欣蕙 Xin-Hui Wu
指導教授: | 潘崇良 Vhorng-Liang Pan
學位類別: | 碩士
學校名稱: | 國立臺灣海洋大學
系所名稱: | 食品科學系
學號: | M94320031
畢業學年度: | 95
語文別: | 中文
關鍵字: | 洋菜酶 agarase
| 酵素純化 enzyme purification
| 酵素生化性質 enzyme characterization
| 新洋菜寡糖 neoagaroligosaccharide
| 水解產物 hydrolysate
| 洋菜降解 agar-degrading
全文點閱次數: | 6

全文影像連結:

論文全文(pdf)

論文頁數: | 115
摘要: | 中文摘要
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| Pseudomonas vesicularis MA103 為一株具有分解洋菜能力的海洋細菌。將此菌於 MMB-MAEF108 發酵槽中26oC、150 rpm、48 hr、pH 6.2、通氣量 4.0 L/min培養，收集離心去除菌體後之培養液，進行洋菜酶的分離與純化。
| 以 DE-52 離子交換樹脂層析，可分離出三個具分解洋菜能力的蛋白質波峰劃分區域，個別收集後命名為 PV-1、PV-2、及 PV-3，其比活性分別為 5008.73 U/mg、5801.76 U/mg、及 6604 U/mg，純化倍數分別為 18.89 倍、20.69 倍、及 23.85 倍。PV-1 及 PV-2 再經 Bio-Gel P-100 膠體過濾層析其純化後，經 12% SDS-PAGE 電泳後得知酵素分子質量分別為 62.9 kDa 及 86.4 kDa
| Agarase PV-1 的最適作用 pH 值為 6.0，最適作用溫度為 40oC，但於 50oC 下反應 15 min 相對活性降至 20.00%。Agarase PV-2 的最適作用 pH 值為 7.0，最適作用溫度為 40oC 或 45oC，於50oC 下反應 15 min 相對活性降至 58.86%。
| Agarases PV-1 與 PV-2 以 HIMEDIATM agar 為基質時有最佳之基質特異性活性，相對 agarose 為基質分解活性分別為 1.43 及 1.24 倍。Agarase PV-2 無法分解馬尾藻 (褐藻) 多醣。
| Agarases PV-1 或 PV-2 個別水解中性洋菜 (agarose) 之水解產物經 HPLC 分析，所得產物各為一介於新洋菜二糖與新洋菜六糖間之寡醣類。利用 agarases PV-1 或 PV-2 個別與新洋菜六糖 (neoagarohexaose) 混合反應，經 HPLC 分析，所得產物各為一大於六糖之寡醣類，故推測 agarases PV-1 與 PV-2 為兼具水解及合成反應活性之酵素。
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| Abstract
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| Pseudomonas vesicularis MA103 is a marine bacterium, which has the ability to degrade agar.  Strain MA103 was grown at 26oC for 48 hr in MMB-MA103 broth with 150 rpm, pH 6.2, air flow rate 4.0 L/min in fermenter.  After removing the cells, collected crude enzymes proceed in the separation and purification of the agarases.
| The chromatograph of the wide enzymes with DE-52 gel achieved the separation of three agarases, they are named PV-1, PV-2, and PV-3 by their elution order in NaCl gradient.  The following properties of these PV-1, PV-2, and PV-3 agarases were: specific activity, 5008.73, 5801.76, and 6604 U/mg; purification fold, 18.89, 20.96, and 23.85 fold; respectively.  Agarases PV-1 and PV-2 were purified further by Bio-Gel P-100 chromatography.  The estimated molecular mass of agarases PV-1 and PV-2 were 62.9 kDa and 86.4 kDa, respectively.
| The optimum pH for agarase PV-1 was found to be 6.0.  The optimum temperature for PV-1 was 40oC, but 80% of the enzyme activity was inactivated in 15 minutes under 50oC.  The optimum pH for agarase PV-2 was found to be 7.0.  The optimum temperature for PV-2 was 40oC or 45oC, but 58.86% of the enzyme activity was inactivated in 15 minutes under 50oC.
| The best substrate specific activities of agarases PV-1 and PV-2 toward HIMEDIATM agar were 1.42 and 1.24 folds digestion activity compare to agarose, respectively.  Agarase PV-2 did not have the capability to degrade Sargassum sp. polysaccharide.
| Hydrolytic products of agarose digested by either agarases PV-1 or PV-2 were analyzed by HPLC, and the results showed that they could be oligosaccharides, which have molecular size between neoagarohexaose and neoagarobiose.  Products of neoagarohexaose reacted with agarases PV-1 and PV-2 were analyzed by HPLC.  The products examined could be oligosaccharides, whose molecular size was larger than neoagarohexaose.  Based on these results, agarases PV-1 and PV-2 are enzymes that could have both hydrolytic and polymeric activitires.
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論文目次: | 目　　錄
|
| 目錄    i
| 圖目錄    v
| 表目錄    viii
| 附錄目錄    ix
| 中文摘要    x
| 英文摘要 (Abstract)    xi
| 壹、前言    1
| 貳、文獻整理    3
| 一、洋菜的構造與特性    3
| 二、洋菜酶生產菌株及其分離來源    3
| 三、洋菜酶對洋菜水解的作用機制    5
| 四、洋菜酶之生化特性    6
| 4-1 溫度和 pH 值    6
| 4-2 分子量    7
| 4-3 金屬離子    8
| 五、海藻寡醣組成成分鑑定    9
| 5-1 薄層層析法 (Thin-layer chromatography, TLC)    9
| 5-2 高效能液相層析法 (High-performance liquid chromatography, HPLC)    10
| 5-3 磁核共振光譜法 (Nuclear magnetic resonance, NMR)    10
| 六、洋菜酶的應用    11
| 6-1 細胞的分離    11
| 6-2    DNA的分離    12
| 6-3 藻類原生質體的製作    12
| 6-4 海藻多醣的生產與黏度降低    13
| 6-5 藻類組成的分析    13
| 6-6 融合瘤細胞的分離    14
| 七、藻類多醣之洋菜酶水解物    14
| 7-1 生理活性    14
| 7-2 應用    15
| 參、實驗設計    18
| 肆、實驗材料與方法    19
| 一、實驗材料    19
| 1-1 實驗菌株    19
| 1-2 試驗藥品    19
| 1-2-1 藥品    19
| 1-2-2 電泳標準品    21
| 1-2-3 培養基組成    22
| 1-2-4 洋菜酶反應基質    22
| 1-2-5    DNS 溶液    23
| 1-2-6 膠過濾緩衝液    23
| 1-2-7    SDS-PAGE    23
| 1-2-8 電泳溶液    24
| 1-3 儀器設備    24
| 二、實驗方法    26
| 2-1 洋菜酶之生產及濃縮    26
| 2-1-1 菌株保存    26
| 2-1-2 菌株活化    26
| 2-1-3 洋菜酶之生產    26
| 2-1-4 洋菜酶之濃縮    27
| 2-2 洋菜酶的活性測定    27
| 2-3 蛋白質的定量    28
| 2-4 洋菜酶的純化分離    28
| 2-4-1    DE-52 離子交換樹脂層析    28
| 2-4-2    Bio-Gel P-100 膠體過濾層析    29
| 2-4-3 純化酵素之命名    29
| 2-5 洋菜酶之生化性質測試    29
| 2-5-1 分子量的鑑定    29
| 2-5-2 基質特異性    30
| 2-5-3 最適作用 pH 值    30
| 2-5-4    pH 值的安定性    30
| 2-5-5 最適作用溫度    30
| 2-5-6 熱安定性    31
| 2-5-7 金屬離子的影響    31
| 2-5-8 儲藏安定性    31
| 2-6 洋菜酶之分解產物的探討    31
| 2-6-1 HPLC 鑑定    31
| 2-7 洋菜酶定序    32
| 2-7-1 內部可能胺基酸序列質譜儀分析    32
| 2-8 統計與分析    32
| 伍、結果與討論    33
| 一、洋菜酶的純化分離    33
| 二、洋菜酶的生化性質    35
| 2-1 最適作用溫度與溫度安定性    35
| 2-2 最適作用 pH 值與 pH 值安定性    38
| 2-3 金屬離子之影響    40
| 2-4 儲藏安定性    41
| 2-5 基質特異性    43
| 2-6 水解產物分析    45
| 2-6-1 MA103 agarases 粗酵素液之水解產物分析    45
| 2-6-2 Agarase PV-1 之水解產物分析    46
| 2-6-3 Agarase PV-2 之水解產物分析    47
| 陸、結論    50
| 柒、參考文獻    52
| 捌、附錄    105
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| 圖目錄
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| 圖一、    MA103 garases 粗酵素DE-52層析圖    64
| 圖二、    P. vesicularis MA103 所產洋菜酶 DE-52 部份純化    之12% SDS-PAGE 圖    65
| 圖三、    Agarase PV-1 之 Bio-Gel P-100 膠體過濾層析圖    66
| 圖四、    P. vesicularis MA103所產agarase PV-1 12% SDS-    PAGE 圖    67
| 圖五、    Agarase PV-2 之 Bio-Gel P-100 膠體過濾層析圖    68
| 圖六、    P. vesicularis MA103所產agarase PV-2 12% SDS-    PAGE圖    69
| 圖七、    MA103 agarases 粗酵素液的最適反應溫度    70
| 圖八、    Agarase PV-1 的最適反應溫度    71
| 圖九、    Agarase PV-2 的最適反應溫度    72
| 圖十、    MA103 agarases 粗酵素液之溫度安定性    73
| 圖十一、  Agarase PV-1之溫度安定性    74
| 圖十二、  Agarase PV-2之溫度安定性    75
| 圖十三、  MA103 agarases 粗酵素液於不同緩衝液中的最適      作用 pH 值    76
| 圖十四、  Agarase PV-1 於不同緩衝液中的最適作用 pH 值    77
| 圖十五、  Agrase PV-2 於不同緩衝液中的最適作用 pH 值    78
| 圖十六、  pH 值對 MA103 agrases 粗酵素液之安定性    79
| 圖十七、  pH 值對 agarase PV-1 之安定性    80
| 圖十八、  pH 值對 agarase PV-2 之安定性    81
| 圖十九、  金屬離子對 MA103 agarases 粗酵素液活性之影響    82
| 圖二十、  金屬離子對 agarase PV-1 活性之影響    83
| 圖二十一、金屬離子對 agarase PV-2 活性之影響    84
| 圖二十二、 MA103 agarases粗酵素液對十二種藻類多醣之        基質特異性    85
| 圖二十三、 Agarase PV-1 對十二種藻類多醣之基質特異性    86
| 圖二十四、 Agarase PV-2 對十二種藻類多醣之基質特異性    87
| 圖二十五、 半乳糖、新洋菜二糖、及新洋菜六糖標準品之          高效能液相層析圖    88
| 圖二十六、 MA103 agarases 粗酵素液與 0.1% agarose 反應      1-4 hr 之高效能液相層析圖    89
| 圖二十七、 MA103 agarases粗酵素液與0.1% neoagarohexaose
| 反應 1-4 hr 之高效能液相層析圖    90
| 圖二十八、 MA103 agarases 粗酵素液與 0.1% neoagarobiose
| 反應 1-4 hr 之高效能液相層析圖    91
| 圖二十九、 MA103 agarases 粗酵素液與 0.1% galactose 反應    1-4 hr 之高效能液相層析圖    92
| 圖三十、  Agarase PV-1 與 0.1% agarose 反應 1-4 hr之高      效能液 相層析圖    93
| 圖三十一、 Agarase PV-1 與 0.1% neoagarohexaose 反應 1-4      hr之高效能液相層析圖    94
| 圖三十二、 Agarase PV-1 與 0.1% neoagarobiose 反應 1-4 hr      之高效能液相層析圖    95
| 圖三十三、 Agarase PV-1 與 0.1% galactose 反應 1-4 hr 之高    效能 液相層析圖    96
| 圖三十四、 Agarase PV-2 與 0.1% agarose 反應 1-4 hr 之高      效能液相層析圖    97
| 圖三十五、 Agarase PV-2 與 0.1% neoagarohexaose 反應 1-4      hr 之高效能液相層析圖    98
| 圖三十六、 Agarase PV-2 與 0.1% neoagarobiose 反應 1-4 hr      之高效能液相層析圖    99
| 圖三十七、 Agarase PV-2 與 0.1% galactose 反應 1-4 hr 之      高效能液相層析圖    100
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| 表目錄
|
| 表一、 Pseudomonas vesicularis MA103 洋菜酶純化表    101
| 表二、 MA103 agarases 粗酵素液儲藏安定性    102
| 表三、 Agarase PV-1 儲藏安定性    103
| 表四、 Agarase PV-2 儲藏安定性    104
|
| 附錄目錄
|
| 附錄一、 Agarose 的基本構造及洋菜酶作用位置    105
| 附錄二、 Agaropectin可能組成基本單位構造    105
| 附錄三、 Agarases 的生產微生物、種類及分離來源    106
| 附錄四、 微生物所產洋菜酶分解洋菜產物    108
| 附錄五、 洋菜酶的生化特性    110
| 附錄六、 Agarase PV-2 胺基酸序列質譜儀比對報告一    112
| 附錄七、 Agarase PV-2 胺基酸序列質譜儀比對報告二    113
| 附錄八、 Agarase PV-2 胺基酸序列質譜儀比對報告三    114
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