:::

| | | | | | 第 | | 筆

詳目顯示區塊

第 5 筆
| (以作者名查詢海洋大學圖書館館藏系統)
| (以作者名查詢全國圖書書目資訊網)
| (以作者名查詢全國博碩士系統)
論文名稱: | Alginate Lyase 之基因轉殖至Lactococcus lactis NZ3900表現及Alginate Lyase所產寡醣產物分析和用於酵母菌發酵生產乙醇之探討
| Study on Cloning and Expression of Pseudomonas vesicularis MA103 Alginate Lyase Gene on Lactococcus lactis NZ3900, and the Analysis of Oligosaccharides Produced by Alginate Lyases, and Their Use on Ethanol Fermentation by Yeasts
研究生: | 楊依珊 Yi-Shan Yang
指導教授: | 潘崇良 Chorng-Liang Pan
學位類別: | 碩士
學校名稱: | 國立臺灣海洋大學
系所名稱: | 食品科學系
學號: | M98320049
畢業學年度: | 99
語文別: | 中文
關鍵字: | 轉殖 Transformation
| 褐藻膠裂解酶 Alginate lyase
| 乳酸菌 Lactic acid bacteria
| 乙醇 Ethanol
全文影像連結: | | (本論文20140729開放校外瀏覽)
| 論文全文(pdf)

論文頁數: | 126
摘要: | 本研究目的在探討alginate lyase rpalIII 之 palIII基因構築至乳酸菌(nisin controlled expression system, NICE) 表現系統之 pNZ8149載體上，再以電穿孔法轉殖至 Lactococcus lactis NZ3900 中，並測試轉殖株 Lc. lactis NZ3900 rpalIII 之質體穩定性及以nisin 誘導下其所產 alginate lyase 之酵素活性變化。並探討菌株 MA103 之crude alginate lyases 降解褐藻膠所得的寡醣產物，以及用P. vesicularis MA103粗酵素液、纖維素酶、和酸水解等製程製備馬尾藻熱萃多醣水解液用於酵母菌發酵生產乙醇產率之變化。
| 乳酸菌之轉殖在目標  pal III基因引子對之兩端分別加入 SpeI 和 XbaI 限制酶切位 (alg-pNZ-for 和 alg-pNZ-rev2)，以 alginate lyase  rpal III 質體為模板經 PCR擴增。成功擴增之 palIII基因以 TA-cloning 方式與 pGEM-T vector 組合之重組載體I轉殖入 E. coli DH5，將此重組載體純化後和乳酸菌載體pNZ8149以SpeI 、 XbaI 限制酶剪切，並透過接合反應形成乳酸菌之重組載體II後，電穿孔轉殖至 Lc. lactis NZ300 中，在 BCP-lactose 洋菜平板上培養基上篩選出呈現 lac+ (菌落周圍為黃色) 之成功之轉殖株。將轉殖成功之重組 palII 基因定序，經比對確認其長度 (1,041 bp) 及核苷酸序列無誤，並命名為Lc. lactis NZ300 rpalIII。測試  Lc. lactis NZ300 rpalIII 於含有 0.5% lactose 之 M17 broth 中連續培養 5 天後，其質體保有率仍可維持在 90% 以上。且以 1 ng/mL 濃度之 nisin 在 30oC 下誘導 3 hr 時呈現較佳的 alginate lyase 胞內酵素活性 (0.095 U/mg protein)，經 SDS-PAGE 分析結果顯示約在 40 kDa 處有被誘導出之蛋白質條帶的產生。
| 使用經 0.3% 市售褐藻膠誘導菌株 MA103 之crude alginate lyases 水解褐藻膠，所得寡醣水解物 (< 3 kDa) 經Sephadex G25 膠體過濾層析 (gel permeation chromatography, GPC)，可收集到 5 個寡醣產物 (peaks A1、A5、A6、A7、與 A8)。以市售 alginate lyase 經相同處理程序後，可得到 3 個寡醣產物 (peaks B3、B4、與B5)。區分之寡醣經薄層層析 (thin layer chromatography, TLC) 以及高效能液相層析 (high performance liquid chromatography, HPLC) 分析後推測兩種水解酵素皆可產生聚合度 (degree of polymerization, dp) 為3 (A6 與 B5) 和 4 (A5 與 B4) 之褐藻膠衍生寡醣。測試寡醣產物之抗氧化活性發現 A7 之寡醣具有最佳的清除 DPPH 自由基率 (62.16%) 和螯合亞鐵離子能力 (86.19%)。
| 最後探討以0.3% 褐藻膠誘導菌株 MA103之crude alginate lyases (6.75-6.83 U/mL) 、 3% 纖維素酶 (88 U/g) 以及0.4 M HCl 降解 5% 馬尾藻熱萃多醣液，所得之酵素水解液用於酵母菌發酵生產乙醇試驗，獲得乙醇最佳產率為13.33%，發酵前後還原醣利用率高達 51.07%。
|
| The aim of this study is using palIII gene from alginate lyase  rpalIII construct on LAB nisin controlled expression (NICE) system vector, pNZ8149, then cloned into Lactococcus lactis NZ3900 by electroporating; and testing the vector stability of Lc. lactis NZ3900 rpalIII and its alginate lyase activity induced by nisin. And investigate the lytic products of alginate from crude alginate lyases of Pseudomonas vesiculars MA103. Also, strain MA103 crude enzyme, cellulase, and acid were used to produce hydrolyzates from polysaccharide by hot water extraction from Sargassum, then using these hydrolyzates to evaluate the yield of ethanol fermentation by yeasts.
| The palIII forward primer encode SpeI restriction site and the reverse primer encode XbaI restriction site. Then using the gene of alginate lyase rpalIII as a template for PCR reaction, and this PCR amplified product as ligated into pGEM-T vector, and then transformed into the competent cells of E. coli DH5α. Then pGEM-T vector, which carrying the cloned palIII gene, was digested with SpeI and XbaI, and ligated into the LAB vector pNZ8149. The recombined plasmid (pNZ8149-palIII) was electroporated into the Lc. lactis NZ3900. The yellow colonies were selected on the BCP lactose agar plates. It was proved that the sequence of the cloned DNA fragments was completely identical to the DNA sequence of the palIII gene (1,041 bp), and the succeed clone was designated as Lc. lactis NZ300 rpalIII. After alignment, Lc. lactis NZ300 rpalIII was tested for the stability of transformed gene by continuously transferring in M17L and M17G broth for 5 days. The results showed that this clone could maintain over 90% cells still bearing the cloned recombinant plasmid. The clones induced by 1 ng/mL nisin at 30oC for 3 hr could obtain higher alginate lyase activity (0.095 U/mg protein), which revealed a 40 kDa protein band by SDS-PAGE.
| While 0.3% alginate was used to induced crude alginate lyases from strain MA103 to digested alginate, and the lytic oligosaccharides (< 3 kDa) were separated by Sephadex G25 gel filtration to obtain peaks A1, A5, A6, A7, and A8. Besides, commercial alginate lyase could get 3 lytic oligosaccharides (peaks B3, B4, and B5) by the same procedures. Both of these two degradation could produce degree of polymerization (dp) 3 (A6 and B5) and dp 4 (A5 and B4) lytic oligosaccharides, which analyzed and determined by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The experiment on antioxidant activity of lytic oligosaccharides revealed peak A7 have better DPPH scavenging effect (62.16%) and Fe2+ chelating effect (86.19%).
| While using the induced crude alginate lyases (6.75-6.83 U/mL) of strain MA103, 3% cellulase (88 U/g), and 0.4 M HCl to hydrolyze 5% hot water polysaccharide extracts of Sargassum, the obtained hydrolyzates were utilized as substrates for ethanol fermentation by yeasts. In this Sargassum bioethanol production experiment, the best ethanol yield rate was 13.33% and the reducing sugar utilization rate was 51.07%.
論文目次: | 目錄    i
| 表目錄    v
| 圖目錄    vi
| 附錄目錄    viii
| 中文摘要    ix
| 英文摘要 (abstract)    xi
| 壹、前言    1
| 貳、文獻整理    3
| .一、褐藻膠的組成與結構    3
| 二、褐藻膠裂解酶 (alginate lyase)    3
| 2-1. 生產菌株及分離來源    3
| 2-2. 生化特性    5
| 2-3. 反應機制    6
| 2-4. 產物種類及其對生理活性影響    6
| 三、褐藻膠裂解酶寡醣產物之應用    7
| 四、褐藻膠裂解酶基因選殖    8
| 五、馬尾藻 (Sargassum sp.)    8
| 5-1. 馬尾藻之來源與應用    8
| 5-2. 馬尾藻之成份與結構組成    9
| 5-3. 海藻產製生質酒精的優勢與開發現況    10
| 六、乳酸菌    11
| 6-1. 菌種    11
| 6-2. 生理活性    11
| 6-3. 基因轉殖之應用    11
| 七、褐藻膠降解後產物分析    12
| 7-1. 薄層層析法 (thin layer chromatography, TLC)    12
| 7-2.高效能液相層析法.(high performance liquid chromatography, HPLC)    13
| 7-3.    傅立葉紅外線光譜分析法 (Fourier transform infrared spectroscopy, FT-IR)    13
| 參、實驗設計    14
| 一、    Alginate lyase rpal III 之 palIII基因選殖至乳酸菌    14
| 二、P. vesicularis MA103 之crude alginate lyases 水解褐藻膠之寡醣產物分析及酵母菌發酵馬尾藻熱萃多醣水解液之探討    15
| 肆、實驗材料與方法    16
| 一、實驗材料    16
| 1-1. 原料    16
| 1-2. 實驗菌株    16
| 1-3. 試驗藥品    16
| 1-3-1. ...藥品    16
| 1-3-2. ...載體    19
| 1-3-3. ...引子    19
| 1-3-4. ...聚合酶鏈鎖反應試劑    19
| 1-3-5. ...限制酶及蛋白酶抑制劑    19
| 1-3-6. ...蛋白質及DNA 翠取純化套裝試劑    19
| 1-3-7. ...電泳標準品    20
| 1-3-8. ...培養基組成    20
| 1-3-9. ...褐藻膠裂解酶反應基質    22
| 1-3-10. 酵素活性反應試劑    22
| 1-3-11. 膠過濾緩衝液    23
| 1-3-12. 電泳膠片配置    23
| 1-3-13. 電泳溶液    24
| 1-3-14. 無菌過濾    25
| 1-3-15. 薄層層析矽膠片    25
| 1-4. 儀器設備    25
| 二、實驗方法    26
| 2-1. 菌株保存與活化    26
| 2-1-1. 菌株保存    26
| 2-1-2. 菌株活化    27
| 2-2.  P. vesicularis MA103 之 palIII 基因轉殖至乳酸菌    28
| 2-2-1.    引子設計    28
| 2-2-2.    擴增目標基因反應(PCR)    28
| 2-2-3.    大腸桿菌之 TA-cloning 轉形作用    29
| 2-2-4.    乳酸菌之電穿孔轉形作用    31
| 2-2-5.    DNA序列比對    33
| 2-2-6.    重組乳酸菌質體穩定性之測試    33
| 2-2-7.    Nisin對重組乳酸菌生長之影響    33
| 2-2-8.    乳酸菌重組 alginate lyase之酵素活性分析    34
| 2-3.  P. vesicularis MA103 之 crude alginate lyases 水解寡醣產物之分析    35
| 2-3-1.  褐藻膠裂解酶水解寡醣產物製備    35
| 2-3-2.  膠體過濾層析分析(GPC)    36
| 2-3-3.  薄層層析分析(TLC)    36
| 2-3-4.  高效能液相層析分析(HPLC)    37
| 2-3-5.  傅立葉紅外線光譜分析(FT-IR)    37
| 2-3-6.  褐藻膠寡醣抗氧化活性分析    37
| 2-4.  馬尾藻熱萃多醣水解液發酵試驗    38
| 2-4-1.  馬尾藻熱萃多醣水解液之製備    38
| 2-4-2.  酵素和酸水解馬尾藻熱萃多醣液    38
| 2-4-3.  馬尾藻熱萃多醣水解液乙醇發酵試驗    39
| 2-4-4.  發酵前後發酵液醣量變化之測定    40
| 2-4-5.  發酵後酒精濃度之氣相層析分析(GC)    40
| 三、統計分析    41
| 伍、結果討論    42
| 一、P. vesicularis MA103 之 palIII 基因轉殖至乳酸菌    42
| 1. 大腸桿菌之 TA-cloning 轉形作用    42
| 2. 乳酸菌之電穿孔轉形作用    42
| 3. 重組乳酸菌質體穩定性之測試    43
| 4.    Nisin對重組乳酸菌生長之影響    44
| 5. 乳酸菌重組 alginate lyase之酵素活性分析    44
| 二、P. vesicularis MA103 之 crude alginate lyases 水解寡醣產物之分析    45
| 1. 膠體過濾層析分析 (GPC)    45
| 2. 薄層層析分析 (TLC)    46
| 3. 高效能液相層析分析 (HPLC)    46
| 4. 傅立葉紅外線光譜分析 (FT-IR)    47
| 5. 褐藻膠寡醣抗氧化活性分析    47
| 三、馬尾藻熱萃多醣水解液發酵生產乙醇試驗    48
| 陸、結論    50
| 柒、參考文獻    51
| 捌、附錄    101
|
參考文獻: | 吳紹祺。1999。海洋菌所產 agarase 生產條件與海洋多醣經 agarase 分解所得寡醣類組成之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 吳紹祺。2005。海洋多醣洋菜酶水解物及其發酵產物之生理活性研究。國立臺灣海洋大學食品科學系博士學位論文，基隆，臺灣。
| 林慧傑。2010。Pseudomonas vescicularis MA103 所產 Alginate lyase pal III 之基因轉殖至大腸桿菌中表現之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 梁鳳鈺。1999。Pseudomonas vesicularis MA103 菌株所產洋菜酶的純化與性質。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 施姵岑。2009。石蓴寡醣之生產與其生理活性之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 黃崇真。2004。腸道微生物與益生菌。食品工業月刊。36: 4-15。
| 陳怡均。2001。Galectin-1 與Thioredoxin peroxidase II 基因之選殖及表現。國立中央大學生命科學系碩士學位論文，桃園，臺灣。
| 陳柏璇。2010。Aeromonas salmonicida MAEF108 之 Agarase AS-IIIb 基因轉殖至乳酸菌及乳酸菌轉殖株發酵山藥乳製品之探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 陳慶源、林富美。2004。益生菌之保健功能。食品工業。36: 1-3。
| 潘崇良、陳衍昌、 蔡震壽、方翠筠、黃意真、鄒文雄、林富邦、劉秀美、唐世杰。2009。海洋功能性醣體研究: 藻類多醣生質乙醇與丁醇及碳交易附加收入。海洋功能性醣體研究: 藻類多醣生質乙醇與丁醇及碳交易附加收入。第三屆海峽兩岸「魚類生理與養殖」研討會，國立臺灣海洋大學水產生物科技頂尖研究中心主辦，行政院國家科學委員會、中央研究院細胞與個體生物學研究所協辦，2009年10月19-21日，基隆，臺灣。
| 潘崇良。2010 。利用藻類生產生質能源。科學發展。448: 26-32。
| 高慶煌。2002。以熱分析法測定基丁質類物質之乙醯化程度。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 許志維。2008。 Aeromonas salmonicida MAEF108 所產 Agarase AS-IIIb 基因轉殖至大腸桿菌與酵母菌及其表現。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 張凱淳。2011。由藍細菌 Synechocystis sp. strain PCC6803 菌株所產藍藻蛋白合成酶之基因在乳酸菌 Nisin 控制的基因表現系統中之表現與藍藻蛋白產量探討。國立臺灣海洋大學食品科學系碩士學位論文，基隆，臺灣。
| 范繼中、李雅琳、吳純衡。2008。海洋綠金－利用海藻生產生質酒精。農政與農情。行政院農業委員會。臺北，臺灣。
| 葉美娟、許家榮。2005。以葡萄醣醛酸酶基因gusA 為報告基因探討 nisin 調控表現系統於乳酸菌 Lactobacillus paracasei 之調控表現研究。台灣農業化學與食品科學。43: 86-96。
| 廖啟成。1998。乳酸菌之分類應用。食品工業。30: 1-10。
| 臺灣海藻資訊網。2004。何謂海藻。http://web.ntm.gov.tw/ seaweeds/search/search_show1.asp?ID=2&SID=104 (June 17, 2011, Taiwan)。
| 賴宜甄。2006。藻類多醣體應用於化妝品之抗老化分析。嘉南藥理科技大學化妝品科技研究所碩士學位論文，嘉義，臺灣。
| Akalin, A. S., Gonc, S., and Duzel, S. 1997. Influence of yogurt and acidophilus yogurt on serum cholesterol levels in mice.  Journal of Dairy Science  80: 2721-2725.
| An, Q. D., Zhang, G. L., Wu, H. T., Zhang, Z. C., Gong, W., Liu, Y., Li, X. Z., and Murata, Y. 2008. Production and partial properties of alginase from newly isolated Flavobacterium sp. LXA. Process Biochemistry  43: 842-847.
| An, Q. D., Zhang, G. L., Wu, H. T., Zhang, Z. C., Zheng, G. S., Luan, L. Murata, Y., and Li, X. 2009. Alginate-deriving oligosaccharide production by alginase from newly isolated Flavobacterium sp. LXA and its potential application in protection against pathogens.  Journal of Applied Microbiology  106: 161-170.
| Arunachalam, K. D.　1999. Role of bifidobacteria in nutrition, medicine and technology.  Nutrition Research  19: 1559-1597.
| Baron, A. J., Wong, T. Y., Hicks, S. J., Gacesa, P., Willcock, D., and McPherson, M. J. 1994. Alginate lyase from Klebsiella pneumoniae subsp. aerogenes: Gene cloning, sequence analysis and high-level production in Escherichia coli.  Gene 143: 61-66.
| Bertani, G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. The Journal of Bacteriology  62: 293-300.
| Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.  Biochemistry  72: 248-254.
| Cao, L., Xie, L., Xue, X., Tan, H., Liu, Y., and Zhou, S. 2007.  Purification and characterization of alginate lyase from Streptomyces sp. strain A5 isolated from banana rhizosphere. Journal of Agricultural and Food Chemistry  55: 5113-5117.
| Cara, C., Ruiz, E., Oliva, J. M., Sáez, F., and Castro, E.  2008. Conversion of olive tree biomass into fermentable sugars by dilute acid pretreatment and enzymatic saccharification. Bioresource Technology  99: 1869-1876.
| Carvalheiro, F., Duarte, L. C., and Gírio, F. M. 2008. Hemicellulose biorefineries: a review on biomass pretreatments. Journal of Scientific  and  Industrial  Research  67: 849-864.
| Chen, H. M., Zheng, L., Lin, W., and Yan, X. J. 2004. Product monitoring and quantitation of oligosaccharides compositeon in agar hydrolyastes by percolumn labeling HPLC.  Talanta  64: 773-777.
| Chynoweth, D. P.  2002.  Review of biomethane from Marine Biomass. Department of Agricultural and Biological Engineering, University of  Florida, Gainesville, F.L, U.S.A.
| Davis, T. A., Lianes, F., Volesky, B., Diaz-Pulido, G., McCook, L., and Mucci, A.  2003.  1H-MNR study of Na alginates extracted from Sargassum spp. in relation of metal biosorption.  Applied Biochemistry and Biotechnology  100: 75-90.
| de Ruyter, P. G., Kuipers, O. P., Beerthuyzen, M. M., Alen-Boerrigter, I., and de Vos, W. M. 1996. Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis. The Journal  of  Bacteriology  178: 3434-3439.
| Dinis, T. C. P., Madeira, V. M. C., and Almeida, L. M.  1994.  Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers.  Archives of Biochemistry and Biophysics  315: 161-169.
| Dubois, M., Gillles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F.  1956.  Colorimetric method for determination of sugars and related substances.  Analytical Chemistry  28: 350-356.
| Ertesvag, H., Erlien, F., Skjak-Bræk, G., Rehm, B. H. A., and Valla, S. 1998. Biochemical properties and substrate specificities of a recombinantly produced Azotobacter vinelandii alginate lyase.  Journal of Bacteriology  180: 3779-3784.
| Federle, M. J., and Bassler, B. L. 2003. Interspecies communication in bacteria.  The Journal of Clinical Investigation  112: 1291-1299.
| Fu, X. T., Lin, H., and Kim, S. M. 2007. Purification and characterization of a Na+/K+ dependent alginate lyase from tuban shell Vibrio sp. YKW-34.  Enzyme and Microbial Technology  41: 828-834.
| Gimmestad, M., Ertesvag, H., Heggeset, T. M. B., Aarstad, O., Svanem, B. I. G., and Valla, S.  2009.  Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination. Journal of Bacteriology 191: 4845-4853.
| Gombotz, W. R., and Wee, S. F.  1998.  Protein release from alginate matrices.  Advance Drug Delivery Reviews  31: 267-285.
| Harris, P.  1990.  Food Gels. Elsevies Science Publishing Co., Inc. New York, U.S.A.
| Haug, A., Larsen, B., and Smidsrod, O.  1966.  A study of the constitution of alginic acid by partial acid hydrolysis.  Acta Chemica Scandinavica  20: 183-190.
| Heyraud, A., and Leonard, C. 1990. Structural characterization of alginates by liquid chromatographies.  Food Hydrocolloids  4: 59-68.
| Heyraud, A., Colin-Morel, P., Girond, S., Richard, C., and Kloareg, B. 1996. HPLC analysis of saturated or unsaturated oligoguluronates and oligomannuronates. Application to the determination of the action pattern of Haliotis tuberculata alginate lyase. Carbohydrate Research  29: 115-126.
| Invitrogen　Corporation. 2002.　pPICZB Manual. http://tools. invitrogen.com/content/sfs/manuals/ppiczalpha_man.pdf (Downloaded in June 15, 2011, USA).
| Iwamoto, T., Araki, R., Iriyama, K. I., Oda, T., Fukuda, H., Hayashida, S., and Muramatsu, T.  2001.  Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No. 272 and its action on saturated oligomeric substrates.  Bioscience Biotechnology and Biochemistry  65: 133-142.
| Iwamoto, Y., Xu, X., Tamura, T., Oda, T., and Muramatsu T. 2003. Enzymatically depolymerized alginate lgomers that cause cytotoxic cytokine production in human mononuclear cells. Bioscience Biotechnology and Biochemistry  67: 258-263.
| Kleerebezem, M., Beerthuyzen, M. M., Vaughan, E. E., Devos, W. M., and Kuipers, O. P. 1997. Controlled gene expression systems for lactic acid bacteria: transferable nisin-inducible expression cassettes for Lactococcus, Leuconostoc, and Lactobacillus spp. Applied and Environmental Microbiology  63: 4581-4584.
| Kootstra, A. M. J., Beeftink, H. H., Scott, E. L., and Sanders, J. P. M. 2009. Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw.  Biochemical Engineering Journal  46: 126-131.
| Kuipers, O. P., de Ruyter, P. G. G. A., Kleerebezem, M., and de Vos, W. M. 1998. Quorum sensing-controlled gene expression in lactic acid bacteria.  The Journal of Bacteriology  64: 15-21.
| Leal, D., Matsuhiro, B., Rossi, M., and Caruso, F. 2008. FT-IR spectra of alginic acid block fractions in three species of brown seaweeds. Carbohydrate Research  343: 30-316.
| Li, X., Dang, X., Zhao, C., Chen, Z., and Chen, F.  2003.  Isolation and some properties of cellulose-degrading Vibrio sp. LX-3 with agar-liquefying ability from soil.  World Journal of Microbiology and Biotechnology  19: 375-379.
| Li, X., Xu, A., Xie, H., Yu, W., Xie, W., and Maa, X.  2010.  Preparation of low molecular weight alginate by hydrogen peroxide depolymerization for tissue engineering.  Carbohydrate Polymers  79: 660-664.
| Liu, G., Yue, L., Chi, Z., Yu, W., Chi, Z., and Madzak, C. 2009. The surface display of the alginate lyase on the cells of Yarrowia lipolytica for hydrolysis of alginate.  Marine Biotechnology  11: 619-626.
| Matsubara, Y., Iwasaki, K., and Muramatsu, T. 1998. Action of poly (-L-guluronate) lyase from Corynebacterium sp. ALY-1 strain on saturated oligoguluronates. Bioscience Biotechnology and Biochemistry  62: 1055-1060.
| Matsubara, Y., Kawada, R., Iwasaki, K., Kimura, Y., Oda, T., and Muramatsu, T. 2000. Cloning and sequence analysis of a gene (aly PG) encoding poly(α-L-guluronate) lyase from Corynebacterium sp. strain ALY-1.  Journal of Bioscience and Bioengineering  89:  199-202.
| Matsushima, R., Danno, H., Uchida, M, Ishihara, K., Suzuki, T., Kaneniwa, M., Ohtsubo, Y., Nagata, Y., and Tsuda, M.  2010.  Analysis of extracellular alginate lyase and its gene from a marine bacterial strain, Pseudoalteromonas atlantica AR06.  Application of Microbiology and Biotechnology  86: 567-576.
| McKay, L. L., Balwin, K. A., and Zottola, E. 1972. Loss of lactose metabolism in lactic streptococci.  Applied Microbiology  23: 1090-1096.
| Mierau, I., and Kleerebezem, M. 2005. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis.  Applied Microbiology and Biotechnology  9: 1-13.
| Mierau, I., Leij, P., van Swam, I., Blommestein, B., Floris, E., Mond, J., and Smid, E. J. 2005. Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE®: The case of lysostaphin.  Microbial Cell Factories  4: 15-24.
| Miller, G. L.  1959.  Use of dinitrosalicylic acid reagent for reagent for deterimination of reduceing sugars.  Analytical Chemisry  31: 426-428
| Min, K. H., Sasaki, S. F., Kashiwabara, Y., Umekawa, M., and Nisizawa, K. 1977. Fine structure of SMG alginate fragment in the light of its degradation by alginate lyases of Pseudomonas sp. Journal Biochemistry  81: 555-562.
| Miyazaki, M., Obata, J., Iwamoto, Y., Oda, T., and Muramatsu, T.  2001.  Calcium-sensitive extracellular poly (-L-guluronate) lyase from a marine bacterium Pseudomonas sp. strain F6: Purification and some properties.  Fisheries Science  67: 956-964.
| Muramatsu, T., Yamada, K., Date, M., and Yoshioka, S. 1993. Action of poly (-D-mannuronate) lyase from Turbo cornutus on oligomeric substrates.  Bioscience Biotechnology and Biochemistry 57:1990-1994.
| Nakada, H. I., and Sweeny, P. C. 1967. Alginic acid degradation by eliminases from abalone hepatopancreas. Journal  of  Biological  Chemistry 242: 845–851.
| Ovalle, R., Soll, C. E., Lim, F., Flanagan, C., Rotunda, T., and Lipke, P. N.  2001. Systematic analysis of oxidative degradation of polysaccharides using PAGE and HPLC-MS.  Carbohydrate Research 330: 131-139.
| Panikkar, R., and Brasch, D. J.  1996.  Composition and block structure of alginates from New Zealand brown seaweeds.  Carbohydrate Research  293: 110-132.
| Parvez, S., Lee, H. C., Kim, D. S., and Kim, H. Y.  2005.  Bile salt hydrolase and cholesterol removal effect by Bifidobacterium bifidum NRRL 1976.  World Journal of Microbiology and Biotechnology  22: 455-459.
| Platteeuw, C., van Alen-Boerrigter, I., van Schalkwijk, S., and de Vos, W. M. 1996. Food-grade cloning and expression system for Lactococcus lactis.  Applied and Environmental Microbiology  62: 1008-1013.
| Preiss, J., and Ashwell, G.  1962.  Alginic acid metabolism in bacteria.  Journal  of  Biological  Chemisrty  237: 309-316.
| Preston, L. A., Bender, C. L., and Schiller, N. L. 2001. Analysis and expression of algL, which encodes alginate lyase in Pseudomonas syringae pv. syringae.  DNA Sequence  12: 455-461.
| Rahman, M. M., Inoue, A., Tanaka, H., and Ojima, T. 2010. Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai.  Biochemistry and Molecular Biology  157: 317-325.
| Reith, J. H., Deurwaarder, E. P., and Curvers, A. P. W. M.  2005.  Bio-offshore large-scale cultivation of seaweed in combination with off-shore windparks in the Nordsea (In Dutch).  Energy Research Center of the Netherlands (ECN). Petten, Netherlands.  ECN- C--05-008.
| Rioux, L. E., Turgeon, S. L., and Beaulieu, M.  2007.  Characterization of polysaccharides extracted from brown seaweeds.  Carbohydrate Polymers  69: 530-537.
| Rose, A. H.  1977.  Scientific basis of alcoholic beverage production.  Economic Microbiology  1: 10-40.
| Rowland, I.  2004.  Probiotics and colorectal cancer risk.  The British Journal　of　Nutrition  91: 805-807.
| Saha, B. C., Iten, L. B., Cotta, M. A., and Wu, Y. V. 2005. Dilute acid pretreatment, enzymaticsaccharification and fermentation of wheat straw to ethanol.  Process  Biochemistry  40: 3693-3700.
| Sambrook, J., and D. W. Russell.  2006.  The Condensed Protocol from Molecular Cloning: A Laboratory Manual.  Cold Spring Harbor Lab. Press, Cold Spring Harbor, NY, U.S.A.  pp. 58-59, 177, 188-192, 243, 718-725, 730, 758-760.
| SAS (Satistical Analytical System). 1999. SAS Use’s: Basic Statistical Analysis. SAS Institute Inc., Cary, NC, U.S.A.
| Sawabe, T., Ohtsuka, M., and Ezura Y.  1997.  Novel alginate lyase from marine bacterium Alteromonas sp. strain H-4.  Carbohydrate Research  304: 69-76.
| Sawabe, T., Takahasi, H., Saeki, H., Niwa, K., and Aono, H. 2007.  Enhanced expression of active recombinant alginate lyase AlyPEEC cloned from a marine bacterium Pseudoalteromonas elyakovii in Escherichia coli by calcium compounds.  Enzyme and Microbial Technology  40: 285-291.
| Shalev E., Battino, S., Weiner, E., Colodner, R., and Keness, Y.  1996.  Ingestion of yogurt containing Lactobacillus acidophilus compared with pasteurized yogurt as a prophylaxis for recurrent candida vaginitis and bacterial vaginosis.  Archives of Family Medicine  5: 593-596.
| Shimada, K., Fujikawa, K., Yahara, K., and Nakamura, T. 1992. Antioxidative properties of xanthan on the antioxidation of soybean oil in cyclodextrinemulsion. Journal of Agricultural and Food Chemistry 40: 945-948.
| South, C. R., Hogsett, D. A. and Lynd, L. R. 1993. Continuous fermentation of cellulosic biomass to ethanol. Applied biochemistry and biotechnology  39: 587-600.
| Suzuki, H., Suzuki, K. I., Inoue, A., and Ojima, T. 2006. A novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner.  Carbohydrate Research  341: 1809-1819.
| Terzaghi, B. E., and Sandine W. E. 1975. Improved medium for lactic streptococci and their bacteriophage.  Applied Microbiology 29: 807-813.
| Torres, M. R., Sousa, A. P. A., Silva Filho, E. A. T., Melo, D. F., Feitosa, J. P. A., de Paula R. C. M., and Lima, M. G. S.  2007.  Extraction and physicochenical characterization of Sargassum vulgare alginate from Brazil.  Carbohydrate Research  342: 2067-2074.
| Twomey, D., Ross, R. P., Ryan, M., Meaney, B., and Hill, C.  2002.  Lantibiotics produced by lactic acid bacteria: Structure, function and applications.  Antonie van Leeuwenhoek  82: 165-185.
| van den Ent, F., and Lowe, J. 2006. RF cloning: A restriction-free method for inserting target genes into plasmids. Journal of Biochemical and  Biophysical Methods  67: 67-74.
| Wang, M. L., Wang J. T., and Choong Y. M.  2004.  Simultaneous quantification of methanol and ethanol in alcoholic beverage using a rapid gas chromatographic method coupling with dual internal standards.  Food Chemistry  86: 609-615.
| Wang, T. Y., Preston, L. A., and Schiller, N. L. 2000. Alginate lyase: Review of major sources and enzyme characterization, structure-function analysis, biological roles, and applications.  Annual Review of Microbiology  54: 289-340.
| Wang, Y., Yu, G-L., Zhao, X., Guan, H-S., and Du, Y-G. 2005. Preparation and structural characterization of several oligoguluronic acids. Chinese Academy of Science  26: 179-183.
| Wang, Y. H., Yu, G. L., Wang, X. M., Lv, Z. H., Zhao, X., Wu, Z. H., and Ji, W. S.  2006a.  Purification and characterization of alginate lyase from marine Vibrio sp. YWA.  Acta Biochimica et Biophysica Sinica  38: 633-638.
| Wang, Y., Han, F., Hu, B., Li, J., and Yu, W.  2006b. In vivo prebiotic properties of alginate oligosaccharides prepared through enzymatic hydrolysis of alginate.  Nutrition Research  26: 597- 603.
| Weissbach, A., and Hurwitz, J. 1958. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B.  Journal  of  Biological  Chemistry  234: 705-709.
| Wells, J. M., and Le Page, R. W. F.  1993. Improved cloning vectors and transformation procedure for Lactococcus lactis.  Journal of Applied Bacteriology  74: 629-636.
| Wollowski, I., Rechkemmer, G., and Pool-Zobel, B. L. 2001.  Protective role of probiotics and prebiotics in colon cancer.  American Journal of Clinical Nutrition  73: 451-455.
| Yamamoto, S., Sahara, T., Sato, D., Kawasaki, K., Ohgiya, S., Inoue, A., and Ojima, T. 2008. Catalytivally important amino acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis.  Enzyme and Microbial Technology  43: 396-402.
| Yamamoto, Y., Kurachi, Maki., Yamaguchi, K., and Oda, T. 2007. Induction of multiple cytokine secretion from RAW264.7 cells by alginate oligosaccharides. Bioscience Biotechnology and Biochemistry  71: 238-241.
| Yang, Z., Li, J. P., and Guan, H. S.  2004. Preparation and characterization of oligomannuronates from alginate degrade by hydrogen peroxide.  Carbohydrate Polymers  58: 115-121.
| Ye, H., Zhou, C., Liu, J., and Zeng, X.  2008. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum.  Food Chemistry  111: 428-432.
| Zhang, Z. Q., Yu, G. L., Z    hao, X., Li, Q., and Guan, H. S.  2005.  Thin layer chromatography analysis of several uronates and their oligosaccharides. Chinese Journal of Analytical Chemistry 33: 1750-1752.
| Zhang, Z., Yu, G., Guan, H., Zhao, X., Dub, Y., and Jiangc, X.  2004. Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510.  Carbohydrate Research 339: 1475-1481.
| Zhao, X., Yu, G., Guan, H., Yue, N., Zhang, Z., and Li, H. 2007. Preparation of low-molecular-weight polyguluronate sulfate and its anticoagulant and anti-inXammatory activities.  Carbohydrate Polymers  69: 272-279.
|

|

預覽及輸出檢索結果

記錄

欄位

| 本頁 1 筆
| 記錄 5 至 43
| 選擇之記錄

| 標題
| 全部欄位
| selected fields new window
| APA Style
| Chicago Style
| MLA Style
| CNS-13611 Style
| EndNote Style

e-mail 地址 | : |
e-mail 標題 | : |

| | |

系統規劃：海洋大學圖書館
系統製作：飛資得資訊有限公司

2011 年 9 月 22 日 20:02