Practical Immunology

Course Introduction

1.     實驗項目內容 龔-含五項-in vitro:

1. ELISA (Antibody standard curve)

2. Hybridoma (adjuvant, cell fusion, HAT selection)

3. Immunohistochemistry (HB4C5, FIA & EIA)

4. Phagocytosis (J774.1, NBT, NO, Yeast stain)

5. Western Blotting (HB4C5, EP-PVDF-BCIP/NBT)

2.     實驗項目內容 吳-含五項-in vivo:

1. mouse immunization

2. anti-serum, titration ELISA

3. immune cell morphology stainning

4. JEV infected cell detection using Ab

5. Flowcytometry